Back to Search
Start Over
Identification of an Interfering Ligand Aptamer for EphB2/3 Receptors.
- Source :
-
Nucleic acid therapeutics [Nucleic Acid Ther] 2016 Apr; Vol. 26 (2), pp. 102-10. Date of Electronic Publication: 2016 Jan 29. - Publication Year :
- 2016
-
Abstract
- The Eph receptors are transmembrane proteins that belong to the receptor tyrosine kinases superfamily. Elevated Eph/ephrin expression levels have been associated with angiogenesis and tumor vasculature in many types of human cancers, including breast, lung, and prostate cancers, melanoma, and leukemia. In glioblastoma (GBM), the dysregulated expression of Eph receptors and of corresponding ephrin ligands has been associated with higher tumor grade and poor prognosis making them effective targets for therapeutic drugs. In this study, we describe the GL43.T, an anti-Eph aptamer, able to bind at high-affinity EphB3 and EphB2. Moreover, the GL43.T aptamer inhibits the glioma cell vitality and interferes with ephrine-B1 inhibition of chemotactic serum-stimulated cell migration. GL43.T aptamer represents a promising therapeutic molecule for EphB3-dependent cancers.
- Subjects :
- A549 Cells
Antineoplastic Agents pharmacology
Aptamers, Nucleotide pharmacology
Base Sequence
Binding Sites
Cell Movement
Cell Proliferation
Drug Screening Assays, Antitumor
Gene Expression
Gene Knockdown Techniques
Humans
RNA Interference
Receptor, EphB2 biosynthesis
Receptor, EphB3 biosynthesis
Aptamers, Nucleotide genetics
Receptor, EphB2 genetics
Receptor, EphB3 genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2159-3345
- Volume :
- 26
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Nucleic acid therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 26824783
- Full Text :
- https://doi.org/10.1089/nat.2015.0580