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Prognostic significance of nuclear or cytoplasmic nucleolin expression in human non-small cell lung cancer and its relationship with DNA-PKcs.
- Source :
-
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine [Tumour Biol] 2016 Aug; Vol. 37 (8), pp. 10349-56. Date of Electronic Publication: 2016 Feb 04. - Publication Year :
- 2016
-
Abstract
- This study investigated the expression of nucleolin in tissue samples in patients with non-small cell lung cancer (NSCLC). Nucleolin was studied to determine whether it has a prognostic value and if its levels correlate with various clinicopathologic parameters. The relationship between nucleolin and expression of DNA-PKcs was also evaluated. Immunohistochemistry was used for detecting the expression levels of nucleolin and DNA-PKcs in tissues from 225 stage IA to IIIB NSCLC patients who underwent lung surgery. Nucleolin was observed predominantly in the cytoplasm, and some levels were observed in the nucleus. Nucleolin expression was higher in NSCLC tissues than adjacent normal lung tissues. Among 225 NSCLC patients, 117 (52.0 %) had high expression of nucleolin. The expression of nucleolin was significantly associated with pathologic stage (P = 0.013) and T status (P = 0.043). Multivariate analysis revealed that nucleolin, cytoplasmic nucleolin, and nuclear nucleolin expression were independent prognostic factors for both overall survival (OS) (P < 0.001) and disease-free survival (DFS) (P < 0.001). A high level of nuclear nucleolin served as an independent prognostic factor for better survival, while a high level of cytoplasmic nucleolin was closely associated with worse prognosis in NSCLC patients. The expression of nucleolin and cytoplasmic nucleolin positively correlated with DNA-PKcs (P < 0.001). These data suggest that nucleolin could be an effective treatment target and prognostic factor for patients with NSCLC.
- Subjects :
- Adult
Aged
Aged, 80 and over
Carcinoma, Non-Small-Cell Lung metabolism
Carcinoma, Non-Small-Cell Lung mortality
Cell Nucleus metabolism
Cytoplasm metabolism
Disease-Free Survival
Female
Humans
Immunohistochemistry
Kaplan-Meier Estimate
Lung Neoplasms metabolism
Lung Neoplasms mortality
Male
Middle Aged
Prognosis
Retrospective Studies
Nucleolin
Carcinoma, Non-Small-Cell Lung pathology
DNA-Activated Protein Kinase metabolism
Lung Neoplasms pathology
Nuclear Proteins metabolism
Phosphoproteins biosynthesis
RNA-Binding Proteins biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0380
- Volume :
- 37
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 26846099
- Full Text :
- https://doi.org/10.1007/s13277-016-4920-6