Back to Search
Start Over
Enhancer of zeste homolog 2 depletion arrests the proliferation of hepatoblastoma cells.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2016 Mar; Vol. 13 (3), pp. 2724-8. Date of Electronic Publication: 2016 Feb 04. - Publication Year :
- 2016
-
Abstract
- Hepatoblastoma is the most common type of malignant liver tumor in children. While outcomes have been greatly improved in the past decades, the treatment of advanced hepatoblastoma has remained challenging. Enhancer of zeste homologue 2 (EZH2), a member of the polycomb group regulators of gene activity, is amplified and overexpressed in a variety of cancers. However, the role of EZH2 in hepatoblastoma has remained to be fully elucidated. The purpose of the present study was to investigate the expression patterns of EZH2 in hepatoblastoma cells and to assess the anti‑cancer effects of EZH2 depletion. Western blot analysis revealed that EZH2 expression was significantly higher in hepatoblastoma specimens compared with that in peri‑tumor tissues, while p27 was reduced in hepatoblastoma. Suppression of EHZ2 using lentiviral small hairpin RNA inhibited hepatoblastoma cell proliferation, induced cell cycle arrest in G1 phase and enhanced the expression of G1/S‑phase checkpoint protein p27. These results suggested that EZH2 may represent a potential diagnostic marker and therapeutic target for the treatment of hepatoblastoma.
- Subjects :
- Child
Cyclin-Dependent Kinase Inhibitor p27 genetics
Cyclin-Dependent Kinase Inhibitor p27 metabolism
Enhancer of Zeste Homolog 2 Protein genetics
Female
G1 Phase Cell Cycle Checkpoints
Gene Expression
Gene Knockdown Techniques
HEK293 Cells
Hep G2 Cells
Hepatoblastoma pathology
Humans
Infant
Liver Neoplasms pathology
Male
RNA, Small Interfering genetics
Cell Proliferation
Enhancer of Zeste Homolog 2 Protein metabolism
Hepatoblastoma metabolism
Liver Neoplasms metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 26848027
- Full Text :
- https://doi.org/10.3892/mmr.2016.4864