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Cyclodextrin-assisted assembly of PEGylated polyester nanoparticles decorated with folate.
- Source :
-
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2016 May 01; Vol. 141, pp. 148-157. Date of Electronic Publication: 2016 Jan 22. - Publication Year :
- 2016
-
Abstract
- In the last decades, nano-oncologicals bearing a polyethylene glycol (PEG) coating are being emerging as biomimetic devices able to drive their drug cargo to solid tumors through passive mechanisms. To improve selectivity toward cancer cells, nanocarriers decorated with the small ligand folate have been widely investigated. Nevertheless, a great challenge remains the effective exposition of folate on nanoparticles (NPs), which is a key prerequisite to ensure the correct binding to receptor and the following endocytic uptake. On these premises, in this study we propose a novel strategy to produce core-shell folate-targeted NPs based on diblock copolymers of poly(ε-caprolactone) (PCL) and PEG through the aid of (2-hydroxypropyl)-β-cyclodextrin (HPβCD). PCL4300-PEG2000 and PCL4300-PEG2000-Fol copolymers were synthesized, characterized and employed to produce NPs without and with HPβCD by a melting/sonication procedure. Colloidal properties of targeted NPs produced with HPβCD demonstrated a highly extended conformation of PEG chains in the shell, an enhanced interaction with a specific antibody against folate and a higher uptake in cells overexpressing folate receptor. Overall, these results suggest that proper manipulation of PEG shell conformation through HPβCD can represent a novel non-covalent strategy to modify shell features.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- A549 Cells
Calorimetry methods
Caproates chemistry
Cell Line, Tumor
Drug Carriers chemistry
Endocytosis
Folate Receptors, GPI-Anchored metabolism
Folic Acid metabolism
Folic Acid pharmacokinetics
Humans
Lactones chemistry
Particle Size
Proton Magnetic Resonance Spectroscopy
Folic Acid chemistry
Nanoparticles chemistry
Polyesters chemistry
Polyethylene Glycols chemistry
beta-Cyclodextrins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4367
- Volume :
- 141
- Database :
- MEDLINE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Publication Type :
- Academic Journal
- Accession number :
- 26852098
- Full Text :
- https://doi.org/10.1016/j.colsurfb.2016.01.035