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Involvement of M1 and CB₁ receptors in the anxiogenic-like effects induced by neostigmine injected into the rat prelimbic medial prefrontal cortex.
- Source :
-
Psychopharmacology [Psychopharmacology (Berl)] 2016 Apr; Vol. 233 (8), pp. 1377-85. Date of Electronic Publication: 2016 Feb 13. - Publication Year :
- 2016
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Abstract
- The prelimbic (PL) medial prefrontal cortex is a brain region highly involved in the control of emotional responses, being modulated by several neurotransmitter systems, including the cholinergic and endocannabinoid. Activation of muscarinic type 1 (M1) receptors in the brain induces retrograde suppression of inhibition through the induction of endocannabinoid release, which, in turn, activates cannabinoid type 1 (CB1) receptors. No study so far, however, has been conducted to investigate if the cholinergic and endocannabinoid systems interact in the PL to modulate anxiety-related behaviors. Thus, the present work aimed at verifying if intra-PL administration of neostigmine, an acetylcholinesterase inhibitor, would produce changes in anxiety-like behavior and if these effects are mediated by M1 and CB1 receptor activation. Independent groups of animals received bilateral injections of vehicle, the M1 receptor antagonist pirenzepine (0.06, 0.6, and 6 nmol), the CB1 receptor antagonist AM251 (0.1 nmol), or the fatty acid amide hydrolase (FAAH) enzyme inhibitor URB597 (1, 3, and 10 pmol), followed by vehicle or neostigmine (0.01, 0.1, and 1 nmol), and were submitted to the elevated plus-maze (EPM) test. Neostigmine (1 nmol) decreased open arm exploration of the maze. This anxiogenic-like effect was reproduced in another anxiety-related animal model, the light-dark box. Previous injection of pirenzepine or AM251 abolished this response in the EPM, whereas URB597 had no effect. These results suggest that CB1 and M1 receptors interact in the PL to control anxiety-like behaviors.
- Subjects :
- Animals
Anxiety chemically induced
Cholinesterase Inhibitors toxicity
Dose-Response Relationship, Drug
Injections, Intraventricular
Male
Muscarinic Antagonists administration & dosage
Neostigmine toxicity
Prefrontal Cortex drug effects
Rats
Rats, Wistar
Receptor, Cannabinoid, CB1 agonists
Receptor, Cannabinoid, CB1 antagonists & inhibitors
Receptor, Muscarinic M1 agonists
Receptor, Muscarinic M1 antagonists & inhibitors
Anxiety metabolism
Cholinesterase Inhibitors administration & dosage
Neostigmine administration & dosage
Prefrontal Cortex metabolism
Receptor, Cannabinoid, CB1 metabolism
Receptor, Muscarinic M1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-2072
- Volume :
- 233
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Psychopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 26873081
- Full Text :
- https://doi.org/10.1007/s00213-016-4228-7