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Genome-wide transcriptional responses of Alteromonas naphthalenivorans SN2 to contaminated seawater and marine tidal flat sediment.
- Source :
-
Scientific reports [Sci Rep] 2016 Feb 18; Vol. 6, pp. 21796. Date of Electronic Publication: 2016 Feb 18. - Publication Year :
- 2016
-
Abstract
- A genome-wide transcriptional analysis of Alteromonas naphthalenivorans SN2 was performed to investigate its ecophysiological behavior in contaminated tidal flats and seawater. The experimental design mimicked these habitats that either added naphthalene or pyruvate; tidal flat-naphthalene (TF-N), tidal flat-pyruvate (TF-P), seawater-naphthalene (SW-N), and seawater-pyruvate (SW-P). The transcriptional profiles clustered by habitat (TF-N/TF-P and SW-N/SW-P), rather than carbon source, suggesting that the former may exert a greater influence on genome-wide expression in strain SN2 than the latter. Metabolic mapping of cDNA reads from strain SN2 based on KEGG pathway showed that metabolic and regulatory genes associated with energy metabolism, translation, and cell motility were highly expressed in all four test conditions, probably highlighting the copiotrophic properties of strain SN2 as an opportunistic marine r-strategist. Differential gene expression analysis revealed that strain SN2 displayed specific cellular responses to environmental variables (tidal flat, seawater, naphthalene, and pyruvate) and exhibited certain ecological fitness traits -- its notable PAH degradation capability in seasonally cold tidal flat might be reflected in elevated expression of stress response and chaperone proteins, while fast growth in nitrogen-deficient and aerobic seawater probably correlated with high expression of glutamine synthetase, enzymes utilizing nitrite/nitrate, and those involved in the removal of reactive oxygen species.
- Subjects :
- Alteromonas drug effects
Alteromonas metabolism
Bacterial Proteins genetics
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial drug effects
Genome, Bacterial
Geologic Sediments microbiology
Metabolic Networks and Pathways
Naphthalenes pharmacology
Pyruvic Acid pharmacology
Seawater microbiology
Water Microbiology
Alteromonas genetics
Geologic Sediments chemistry
Seawater chemistry
Transcriptome
Water Pollutants, Chemical pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26887987
- Full Text :
- https://doi.org/10.1038/srep21796