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Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA.
- Source :
-
Cell reports [Cell Rep] 2016 Mar 01; Vol. 14 (8), pp. 2003-16. Date of Electronic Publication: 2016 Feb 18. - Publication Year :
- 2016
-
Abstract
- Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood. Here, we investigate the role of the Smc ATPase activity in the recruitment of Smc/ScpAB to the Bacillus subtilis chromosome. We demonstrate that targeting of Smc/ScpAB to ParB/parS loading sites is strictly dependent on engagement of Smc head domains and relies on an open organization of the Smc coiled coils. We find that dimerization of the Smc hinge domain stabilizes closed Smc rods and hinders head engagement as well as chromosomal targeting. Conversely, the ScpAB sub-complex promotes head engagement and Smc rod opening and thereby facilitates recruitment of Smc to parS sites. Upon ATP hydrolysis, Smc/ScpAB is released from loading sites and relocates within the chromosome-presumably through translocation along DNA double helices. Our findings define an intermediate state in the process of chromosome organization by Smc.<br /> (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adenosine Triphosphate metabolism
Amino Acid Sequence
Bacillus subtilis genetics
Bacterial Proteins genetics
Cell Cycle Proteins genetics
Cell Division
Chromosome Segregation
Chromosomes, Bacterial ultrastructure
DNA Primase genetics
DNA, Bacterial genetics
Hydrolysis
Isoenzymes genetics
Isoenzymes metabolism
Models, Molecular
Molecular Sequence Data
Protein Domains
Protein Multimerization
Protein Structure, Secondary
Protein Transport
Sequence Alignment
Bacillus subtilis metabolism
Bacterial Proteins metabolism
Cell Cycle Proteins metabolism
Chromosomes, Bacterial chemistry
DNA Primase metabolism
DNA, Bacterial metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 14
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 26904953
- Full Text :
- https://doi.org/10.1016/j.celrep.2016.01.066