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Antibiofilm action of a toluidine blue O-silver nanoparticle conjugate on Streptococcus mutans: a mechanism of type I photodynamic therapy.
- Source :
-
Biofouling [Biofouling] 2016; Vol. 32 (3), pp. 313-28. - Publication Year :
- 2016
-
Abstract
- The objective of this study was to evaluate the anti-biofilm efficacy of photodynamic therapy by conjugating a photosensitizer (TBO) with silver nanoparticles (AgNP). Streptococcus mutans was exposed to laser light (630 nm) for 70 s (9.1 J cm(-2)) in the presence of a toluidine blue O-silver nanoparticle conjugate (TBO-AgNP). The results showed a reduction in the viability of bacterial cells by 4 log10. The crystal violet assay, confocal laser scanning microscopy and scanning electron microscopy revealed that the TBO-AgNP conjugates inhibited biofilm formation, increased the uptake of propidium iodide and leakage of the cellular constituents, respectively. Fluorescence spectroscopic studies confirmed the generation of OH(•) as a major reactive oxygen species, indicating type I phototoxicity. Both the conjugates down-regulated the expression of biofilm related genes compared to TBO alone. Hence TBO-AgNP conjugates were found to be more phototoxic against S. mutans biofilm than TBO alone.
- Subjects :
- Anti-Bacterial Agents pharmacology
Humans
Metal Nanoparticles therapeutic use
Microscopy, Electron, Scanning methods
Photosensitizing Agents pharmacology
Biofilms drug effects
Biofilms growth & development
Dental Caries microbiology
Dental Caries therapy
Photochemotherapy methods
Silver pharmacology
Streptococcus mutans drug effects
Streptococcus mutans physiology
Tolonium Chloride pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1029-2454
- Volume :
- 32
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biofouling
- Publication Type :
- Academic Journal
- Accession number :
- 26905507
- Full Text :
- https://doi.org/10.1080/08927014.2016.1141899