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Nanoparticles based on phenylalanine ethyl ester-alginate conjugate as vitamin B2 delivery system.
- Source :
-
Journal of biomaterials applications [J Biomater Appl] 2016 Jul; Vol. 31 (1), pp. 13-22. Date of Electronic Publication: 2016 Feb 24. - Publication Year :
- 2016
-
Abstract
- Phenylalanine ethyl ester (PAE)-alginate (Alg) conjugate (PAE-Alg, PEA) was synthesized and formation of an amide bond between PAE and Alg was confirmed by Fourier transformed-infrared and (1)H nuclear magnetic resonance spectroscopy. The degree of PAE substitution was 3.5-4.7 (PAE group per hundred sugar residues of Alg) which was determined by elemental analysis. The critical aggregation concentration values determined for PEA conjugates PEA1, PEA2, and PEA3 were 0.20, 0.12, and 0.10 mg/ml, respectively. The particle size of PEA nanoparticles (PEA-NPs) decreased from 425 nm to 226 nm with the increasing degree of PAE substitution. Vitamin B2 (VB2), as a model nutrient, was encapsulated into the nanoparticles. The drug-loading content increased with increasing degree of PAE substitution. The maximum VB2 loading capacity and loading efficiency of PEA3 nanoparticles were 3.53 ± 0.03% and 91.48 ± 0.80%, respectively. The in vitro release behavior of VB2 from the PEA-NPs showed a biphasic release profile with an initial burst release of about 40-50% of VB2 in the first 10 h followed by a steady and continuous release phase for the following 50 h in PBS, pH 7.4. The human colorectal carcinoma cell line was used to investigate the cytotoxicity of PEA-NPs. Our results showed that various concentrations of nanoparticles did not cause significant cytotoxicity against cell lines at normal concentrations.<br /> (© The Author(s) 2016.)
- Subjects :
- Alginates toxicity
Caco-2 Cells
Cell Survival drug effects
Delayed-Action Preparations chemistry
Delayed-Action Preparations toxicity
Diffusion
Drug Delivery Systems standards
Glucuronic Acid chemistry
Glucuronic Acid toxicity
Hexuronic Acids chemistry
Hexuronic Acids toxicity
Humans
Kinetics
Materials Testing
Nanocapsules toxicity
Nanocapsules ultrastructure
Nanoconjugates chemistry
Nanoconjugates toxicity
Nanoconjugates ultrastructure
Particle Size
Phenylalanine chemistry
Phenylalanine toxicity
Riboflavin chemistry
Alginates chemistry
Delayed-Action Preparations administration & dosage
Drug Delivery Systems methods
Nanocapsules chemistry
Nanoconjugates administration & dosage
Phenylalanine analogs & derivatives
Riboflavin administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1530-8022
- Volume :
- 31
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of biomaterials applications
- Publication Type :
- Academic Journal
- Accession number :
- 26916950
- Full Text :
- https://doi.org/10.1177/0885328216630497