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'Smelling' the cerebrospinal fluid: olfactory signaling molecules are expressed in and mediate chemosensory signaling from the choroid plexus.
- Source :
-
The FEBS journal [FEBS J] 2016 May; Vol. 283 (9), pp. 1748-66. Date of Electronic Publication: 2016 Mar 30. - Publication Year :
- 2016
-
Abstract
- The olfactory-type signaling machinery has been known to be involved not only in odorant detection but also in other tissues with unsuspected sensory roles. As a barrier, the choroid plexus (CP) is an active participant in the monitoring of the cerebrospinal fluid (CSF), promptly responding to alterations in its composition. We hypothesized that olfactory signaling could be active in CP, contributing to the surveillance of the CSF composition. We determined the mRNA and protein expression of the major components of the olfactory transduction pathway in the rat CP, including odorant receptors, the olfactory G-protein (Gαolf), adenylate cyclase 3 and cyclic nucleotide-gated channel 2. The functionality of the transduction pathway and the intracellular mechanisms involved were analyzed by DC field potential recording electrophysiological analysis, in an ex vivo CP-brain setup, using polyamines as stimuli and blockers of the downstream signaling pathways. Concentration-dependent responses were obtained for the polyamines studied (cadaverine, putrescine, spermine and spermidine), all known to be present in the CSF. Transfection of a CP epithelial cell line with siRNA against Gαolf effectively knocked down protein expression and reduced the CP cells' response to spermine. Thus, the key components of the olfactory chemosensory apparatus are present and are functional in murine CP, and polyamines seem to trigger both the cAMP and the phospholipase C-inositol 1,4,5-trisphosphate pathways. Olfactory-like chemosensory signaling may be an essential component of the CP chemical surveillance apparatus to detect alterations in the CSF composition, and to elicit responses to modulate and maintain brain homeostasis.<br /> (© 2016 Federation of European Biochemical Societies.)
- Subjects :
- Adenylyl Cyclases cerebrospinal fluid
Animals
Cadaverine cerebrospinal fluid
Cadaverine pharmacology
Cell Line
Choroid Plexus cytology
Choroid Plexus drug effects
Cyclic AMP cerebrospinal fluid
Cyclic Nucleotide-Gated Cation Channels antagonists & inhibitors
Cyclic Nucleotide-Gated Cation Channels cerebrospinal fluid
Epithelial Cells cytology
Epithelial Cells drug effects
GTP-Binding Protein alpha Subunits antagonists & inhibitors
GTP-Binding Protein alpha Subunits cerebrospinal fluid
Gene Expression Regulation
Inositol 1,4,5-Trisphosphate cerebrospinal fluid
Olfactory Pathways physiology
Olfactory Perception physiology
Polyamines cerebrospinal fluid
Polyamines pharmacology
Primary Cell Culture
Putrescine cerebrospinal fluid
Putrescine pharmacology
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Rats
Rats, Wistar
Signal Transduction
Spermidine cerebrospinal fluid
Spermidine pharmacology
Spermine cerebrospinal fluid
Spermine pharmacology
Type C Phospholipases cerebrospinal fluid
Type C Phospholipases genetics
Adenylyl Cyclases genetics
Choroid Plexus metabolism
Cyclic Nucleotide-Gated Cation Channels genetics
Epithelial Cells metabolism
GTP-Binding Protein alpha Subunits genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1742-4658
- Volume :
- 283
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The FEBS journal
- Publication Type :
- Academic Journal
- Accession number :
- 26934374
- Full Text :
- https://doi.org/10.1111/febs.13700