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Titin antibodies in "seronegative" myasthenia gravis--A new role for an old antigen.
- Source :
-
Journal of neuroimmunology [J Neuroimmunol] 2016 Mar 15; Vol. 292, pp. 108-15. Date of Electronic Publication: 2016 Jan 27. - Publication Year :
- 2016
-
Abstract
- Myasthenia gravis (MG) is an autoimmune disease caused by antibodies targeting the neuromuscular junction of skeletal muscles. Triple-seronegative MG (tSN-MG, without detectable AChR, MuSK and LRP4 antibodies), which accounts for ~10% of MG patients, presents a serious gap in MG diagnosis and complicates differential diagnosis of similar disorders. Several AChR antibody positive patients (AChR-MG) also have antibodies against titin, usually detected by ELISA. We have developed a very sensitive radioimmunoprecipitation assay (RIPA) for titin antibodies, by which many previously negative samples were found positive, including several from tSN-MG patients. The validity of the RIPA results was confirmed by western blots. Using this RIPA we screened 667 MG sera from 13 countries; as expected, AChR-MG patients had the highest frequency of titin antibodies (40.9%), while MuSK-MG and LRP4-MG patients were positive in 14.6% and 16.4% respectively. Most importantly, 13.4% (50/372) of the tSN-MG patients were also titin antibody positive. None of the 121 healthy controls or the 90 myopathy patients, and only 3.6% (7/193) of other neurological disease patients were positive. We thus propose that the present titin antibody RIPA is a useful tool for serological MG diagnosis of tSN patients.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Enzyme-Linked Immunosorbent Assay
Female
Humans
International Cooperation
LDL-Receptor Related Proteins immunology
Male
Myasthenia Gravis epidemiology
Radioimmunoprecipitation Assay
Receptor Protein-Tyrosine Kinases immunology
Receptors, Cholinergic immunology
Autoantibodies blood
Connectin immunology
Myasthenia Gravis blood
Myasthenia Gravis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8421
- Volume :
- 292
- Database :
- MEDLINE
- Journal :
- Journal of neuroimmunology
- Publication Type :
- Academic Journal
- Accession number :
- 26943968
- Full Text :
- https://doi.org/10.1016/j.jneuroim.2016.01.018