Back to Search
Start Over
Expression and potential roles of IL-33/ST2 in the immune regulation during Clonorchis sinensis infection.
- Source :
-
Parasitology research [Parasitol Res] 2016 Jun; Vol. 115 (6), pp. 2299-305. Date of Electronic Publication: 2016 Mar 05. - Publication Year :
- 2016
-
Abstract
- During clonorchiasis, immune responses of hosts are responsible for the removal of the worms and also are involved in the progress of the pathological damage caused by Clonorchis sinensis. Interleukin-33 (IL-33), a recently described cytokine signaling through the ST2 receptor, has emerged as a potent inducer to bile duct proliferation and fibrosis; however, little is known of this signaling in the pathogen-caused periductal inflammation and fibrosis. In the present study, using immunohistochemistry, real-time PCR, enzyme-linked immunosorbent assay (ELISA), and flow cytometry, we studied the expression of IL-33/ST2 during C. sinensis infection, as well as their potential roles in C. sinensis-induced host immune responses. The results showed that a higher level of IL-33 was detected in the sera of patients of clonorchiasis (nā=ā45), compared with in those of healthy donors (nā=ā16). Similarly, in FVB mice experimentally infected with C. sinensis, a higher level of IL-33 was detected at latent stage both in the serum and in the liver, as well as the up-regulated expression of ST2 receptor on the inflammatory cells, especially on CD4(+) T cells in the liver of infected mice. Our results, for the first time, indicated that the increased IL-33/ST2 may be involved in the regulation of immunopathology induced by C. sinensis.
- Subjects :
- Animals
Bile Ducts pathology
CD4-Positive T-Lymphocytes immunology
Clonorchiasis blood
Clonorchiasis parasitology
Clonorchis sinensis pathogenicity
Enzyme-Linked Immunosorbent Assay veterinary
Female
Fibrosis pathology
Flow Cytometry
Humans
Liver pathology
Mice
Real-Time Polymerase Chain Reaction
Clonorchiasis immunology
Clonorchis sinensis immunology
Interleukin-1 Receptor-Like 1 Protein metabolism
Interleukin-33 blood
Interleukin-33 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1955
- Volume :
- 115
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 26944417
- Full Text :
- https://doi.org/10.1007/s00436-016-4974-9