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Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing.
Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2016 Mar 22; Vol. 113 (12), pp. E1625-34. Date of Electronic Publication: 2016 Mar 08. - Publication Year :
- 2016
-
Abstract
- C/D box small nucleolar RNAs (SNORDs) are small noncoding RNAs, and their best-understood function is to target the methyltransferase fibrillarin to rRNA (for example, SNORD27 performs 2'-O-methylation of A27 in 18S rRNA). Unexpectedly, we found a subset of SNORDs, including SNORD27, in soluble nuclear extract made under native conditions, where fibrillarin was not detected, indicating that a fraction of the SNORD27 RNA likely forms a protein complex different from canonical snoRNAs found in the insoluble nuclear fraction. As part of this previously unidentified complex,SNORD27 regulates the alternative splicing of the transcription factor E2F7p re-mRNA through direct RNA-RNA interaction without methylating the RNA, likely by competing with U1 small nuclear ribonucleoprotein (snRNP). Furthermore, knockdown of SNORD27 activates previously "silent" exons in several other genes through base complementarity across the entire SNORD27 sequence, not just the antisense boxes. Thus, some SNORDs likely function in both rRNA and pre-mRNA processing, which increases the repertoire of splicing regulators and links both processes.
- Subjects :
- Base Pairing
Base Sequence
Cell Cycle
Cell Division
Cell Fractionation methods
Cell Nucleus chemistry
Chromosomal Proteins, Non-Histone analysis
E2F7 Transcription Factor genetics
Exons genetics
Gene Knockdown Techniques
HeLa Cells
Humans
Methylation
Molecular Sequence Data
Oligonucleotides, Antisense genetics
Organelle Biogenesis
Ribonucleoprotein, U1 Small Nuclear metabolism
Ribosomes metabolism
Solubility
Spliceosomes metabolism
Alternative Splicing
RNA Precursors metabolism
RNA Processing, Post-Transcriptional physiology
RNA, Ribosomal metabolism
RNA, Small Nucleolar physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 113
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 26957605
- Full Text :
- https://doi.org/10.1073/pnas.1519292113