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Inflammatory cells, ceramides, and expression of proteases in perivascular adipose tissue adjacent to human abdominal aortic aneurysms.
- Source :
-
Journal of vascular surgery [J Vasc Surg] 2017 Apr; Vol. 65 (4), pp. 1171-1179.e1. Date of Electronic Publication: 2016 Mar 06. - Publication Year :
- 2017
-
Abstract
- Background: Abdominal aortic aneurysm (AAA) is a deadly irreversible weakening and distension of the abdominal aortic wall. The pathogenesis of AAA remains poorly understood. Investigation into the physical and molecular characteristics of perivascular adipose tissue (PVAT) adjacent to AAA has not been done before and is the purpose of this study.<br />Methods and Results: Human aortae, periaortic PVAT, and fat surrounding peripheral arteries were collected from patients undergoing elective surgical repair of AAA. Control aortas were obtained from recently deceased healthy organ donors with no known arterial disease. Aorta and PVAT was found in AAA to larger extent compared with control aortas. Immunohistochemistry revealed neutrophils, macrophages, mast cells, and T-cells surrounding necrotic adipocytes. Gene expression analysis showed that neutrophils, mast cells, and T-cells were found to be increased in PVAT compared with AAA as well as cathepsin K and S. The concentration of ceramides in PVAT was determined using mass spectrometry and correlated with content of T-cells in the PVAT.<br />Conclusions: Our results suggest a role for abnormal necrotic, inflamed, proteolytic adipose tissue to the adjacent aneurysmal aortic wall in ongoing vascular damage.<br /> (Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Adipocytes immunology
Adipocytes pathology
Adipose Tissue immunology
Adipose Tissue pathology
Antigens, CD analysis
Antigens, CD genetics
Aortic Aneurysm, Abdominal genetics
Aortic Aneurysm, Abdominal immunology
Aortic Aneurysm, Abdominal pathology
Cell Adhesion Molecules analysis
Cell Adhesion Molecules genetics
Cytokines analysis
Cytokines genetics
GPI-Linked Proteins analysis
GPI-Linked Proteins genetics
Gene Expression Regulation, Enzymologic
Humans
Macrophages enzymology
Macrophages immunology
Macrophages pathology
Mast Cells immunology
Mast Cells pathology
Necrosis
Neutrophils immunology
Neutrophils pathology
Peptide Hydrolases genetics
T-Lymphocytes immunology
T-Lymphocytes pathology
Adipocytes enzymology
Adipose Tissue enzymology
Aortic Aneurysm, Abdominal enzymology
Ceramides analysis
Mast Cells enzymology
Neutrophils enzymology
Peptide Hydrolases analysis
T-Lymphocytes enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-6809
- Volume :
- 65
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of vascular surgery
- Publication Type :
- Academic Journal
- Accession number :
- 26960947
- Full Text :
- https://doi.org/10.1016/j.jvs.2015.12.056