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Phthalates Are Metabolised by Primary Thyroid Cell Cultures but Have Limited Influence on Selected Thyroid Cell Functions In Vitro.
- Source :
-
PloS one [PLoS One] 2016 Mar 17; Vol. 11 (3), pp. e0151192. Date of Electronic Publication: 2016 Mar 17 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Phthalates are plasticisers added to a wide variety of products, resulting in measurable exposure of humans. They are suspected to disrupt the thyroid axis as epidemiological studies suggest an influence on the peripheral thyroid hormone concentration. The mechanism is still unknown as only few in vitro studies within this area exist. The aim of the present study was to investigate the influence of three phthalate diesters (di-ethyl phthalate, di-n-butyl phthalate (DnBP), di-(2-ethylhexyl) phthalate (DEHP)) and two monoesters (mono-n-butyl phthalate and mono-(2-ethylhexyl) phthalate (MEHP)) on the differentiated function of primary human thyroid cell cultures. Also, the kinetics of phthalate metabolism were investigated. DEHP and its monoester, MEHP, both had an inhibitory influence on 3'-5'-cyclic adenosine monophosphate secretion from the cells, and MEHP also on thyroglobulin (Tg) secretion from the cells. Results of the lactate dehydrogenase-measurements indicated that the MEHP-mediated influence was caused by cell death. No influence on gene expression of thyroid specific genes (Tg, thyroid peroxidase, sodium iodine symporter and thyroid stimulating hormone receptor) by any of the investigated diesters could be demonstrated. All phthalate diesters were metabolised to the respective monoester, however with a fall in efficiency for high concentrations of the larger diesters DnBP and DEHP. In conclusion, human thyroid cells were able to metabolise phthalates but this phthalate-exposure did not appear to substantially influence selected functions of these cells.
- Subjects :
- Cells, Cultured
Cyclic AMP metabolism
Dibutyl Phthalate toxicity
Diethylhexyl Phthalate toxicity
Humans
Phthalic Acids toxicity
Plasticizers toxicity
Thyroglobulin metabolism
Thyroid Gland cytology
Dibutyl Phthalate metabolism
Diethylhexyl Phthalate metabolism
Phthalic Acids metabolism
Plasticizers metabolism
Thyroid Gland drug effects
Thyroid Gland metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 26985823
- Full Text :
- https://doi.org/10.1371/journal.pone.0151192