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[Assessment of applicability of archived cytological lung cancer specimens for molecular genetic analysis].
- Source :
-
Voprosy onkologii [Vopr Onkol] 2015; Vol. 61 (6), pp. 968-71. - Publication Year :
- 2015
-
Abstract
- Molecular genetic analysis of lung tumors is often essential for the proper choice of therapy. EGFR mutation is a well-known marker of sensitivity to gefitinib, erlotinib and afatinib; ALK-translocations make tumor sensitive to several ALK inhibitors; low intratumoral expression of DNA repair genes (ERCC1, BRCA1, etc.) may increase the therapeutic index of platinum-based drugs. Usually these markers are evaluated using formalin-fixed paraffin-embedded tumor tissues. The goal of this work was to assess utility of archived cytological lung cancer specimens as an alternative source of material for molecular genetic testing. We analyzed paired histological and cytological lung adenocarcinoma specimens. Comparison of results within the pairs showed that cytological material can be used instead of histological material for qualitative analyses (detection of EGFR mutations or ALK-translocations); however, gene expression measurements, obtained by quantitative real-time PCR, may differ significantly in histological and cytological samples from the same patient.
- Subjects :
- Adenocarcinoma of Lung
Anaplastic Lymphoma Kinase
DNA Repair drug effects
DNA Repair genetics
Gene Expression Regulation, Neoplastic drug effects
Humans
Molecular Targeted Therapy
Real-Time Polymerase Chain Reaction
Russia
Specimen Handling
Adenocarcinoma genetics
Antineoplastic Agents pharmacology
Biological Specimen Banks
ErbB Receptors genetics
Genetic Testing
Lung Neoplasms genetics
Molecular Biology
Mutation
Receptor Protein-Tyrosine Kinases genetics
Translocation, Genetic
Subjects
Details
- Language :
- Russian
- ISSN :
- 0507-3758
- Volume :
- 61
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Voprosy onkologii
- Publication Type :
- Academic Journal
- Accession number :
- 26995989