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Combination of simvastatin, calcium silicate/gypsum, and gelatin and bone regeneration in rabbit calvarial defects.
- Source :
-
Scientific reports [Sci Rep] 2016 Mar 21; Vol. 6, pp. 23422. Date of Electronic Publication: 2016 Mar 21. - Publication Year :
- 2016
-
Abstract
- The present study was performed to determine whether simvastatin improves bone regeneration when combined with calcium silicate/gypsum and gelatin (CS-GEL). The surface morphology was determined using field-emission scanning electron microscopy (FSEM). Degradation in vitro was evaluated by monitoring the weight change of the composites soaked in phosphate buffered saline (PBS). Drug release was evaluated using high-performance liquid chromatography (HPLC). Cytotoxicity testing was performed to assess the biocompatibility of composites. Four 5 mm-diameter bone defects were created in rabbit calvaria. Three sites were filled with CS-GEL, 0.5 mg simvastatin-loaded CS-GEL (SIM-0.5) and 1.0 mg simvastatin-loaded CS-GEL (SIM-1.0), respectively, and the fourth was left empty as the control group. Micro-computed tomography (micro-CT) and histological analysis were carried out at 4 and 12 weeks postoperatively. The composites all exhibited three-dimensional structures and showed the residue with nearly 80% after 4 weeks of immersion. Drug release was explosive on the first day and then the release rate remained stable. The composites did not induce any cytotoxicity. The results in vivo demonstrated that the new bone formation and the expressions of BMP-2, OC and type I collagen were improved in the simvastatin-loaded CS-GEL group. It was concluded that the simvastatin-loaded CS-GEL may improve bone regeneration.
- Subjects :
- Animals
Bone Substitutes pharmacokinetics
Calcium Compounds pharmacokinetics
Calcium Sulfate pharmacokinetics
Gelatin pharmacokinetics
Male
Materials Testing
Rabbits
Silicates pharmacokinetics
Simvastatin pharmacokinetics
Skull injuries
Skull ultrastructure
Bone Regeneration drug effects
Bone Substitutes administration & dosage
Calcium Compounds administration & dosage
Calcium Sulfate administration & dosage
Gelatin administration & dosage
Silicates administration & dosage
Simvastatin administration & dosage
Skull drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 26996657
- Full Text :
- https://doi.org/10.1038/srep23422