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Rapid and sensitive monitoring of biocatalytic reactions using ion mobility mass spectrometry.
- Source :
-
The Analyst [Analyst] 2016 Apr 21; Vol. 141 (8), pp. 2351-5. Date of Electronic Publication: 2016 Mar 21. - Publication Year :
- 2016
-
Abstract
- The combination of stable isotope labelling with direct infusion ion mobility mass spectrometry (IM-MS) enabled qualitative and quantitative monitoring of biocatalytic reactions with reduced analysis times, enhanced sensitivity and μL-level assay volumes. The new approach was demonstrated by applying to both lipase and monooxygenase enzymes, including multi-substrate screening.
Details
- Language :
- English
- ISSN :
- 1364-5528
- Volume :
- 141
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- The Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 26999769
- Full Text :
- https://doi.org/10.1039/c6an00617e