Back to Search
Start Over
PIK3CA promotes proliferation and motility but is unassociated with lymph node metastasis or prognosis in esophageal squamous cell carcinoma.
- Source :
-
Human pathology [Hum Pathol] 2016 Jul; Vol. 53, pp. 121-9. Date of Electronic Publication: 2016 Mar 18. - Publication Year :
- 2016
-
Abstract
- The PIK3CA mutation has been extensively reported in the setting of cancers; however, the clinicopathological significance of PIK3CA expression has rarely been discussed in esophageal squamous cell carcinoma. In the present study, to confirm the significance of PIK3CA expression in association with metastasis and prognosis, which has been somewhat controversial in esophageal squamous cell carcinoma (ESCC), the relationship between clinicopathological features of ESCC and PIK3CA expression was analyzed using immunohistochemistry with a tissue microarray. Meanwhile, as additional verification and an ethnic control, another independent small cohort of Kazakh ESCC were analyzed by immunohistochemistry. To investigate the pilot role of PIK3CA in ESCC cells, ESCC cell lines ECa109 and EC9706 were transiently transfected with specific siRNA against PIK3CA. The silencing effect was detected by Western blot. Cell proliferation was examined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay; apoptosis and the cell cycles were analyzed by flow cytometry. Furthermore, the migratory and invasive ability were evaluated by wound healing and transwell invasion assay, respectively. Expression of PIK3CA was significantly higher in ESCC than in paired normal controls and was ethnicity independent; no statistically significant difference was observed between PIK3CA expression and sex, age, depth of invasion, tumor differentiation, lymph node metastasis, or prognosis. Proliferation, migration, and invasion were all markedly reduced after knockout of PIK3CA. Moreover, the cell cycle was arrested at the S phase, and the apoptosis rate was significantly increased, suggesting that PIK3CA plays a key role in promoting the proliferation and motility of ESCC cells.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
Aged
Apoptosis
Asian People
Biomarkers, Tumor genetics
Carcinoma, Squamous Cell ethnology
Carcinoma, Squamous Cell genetics
Carcinoma, Squamous Cell secondary
Cell Line, Tumor
China epidemiology
Class I Phosphatidylinositol 3-Kinases
Esophageal Neoplasms ethnology
Esophageal Neoplasms genetics
Esophageal Neoplasms pathology
Esophageal Squamous Cell Carcinoma
Female
Gene Expression Regulation, Neoplastic
Humans
Kaplan-Meier Estimate
Lymphatic Metastasis
Male
Middle Aged
Phosphatidylinositol 3-Kinases genetics
RNA Interference
S Phase Cell Cycle Checkpoints
Signal Transduction
Time Factors
Transfection
Up-Regulation
Biomarkers, Tumor metabolism
Carcinoma, Squamous Cell enzymology
Cell Movement
Cell Proliferation
Esophageal Neoplasms enzymology
Phosphatidylinositol 3-Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-8392
- Volume :
- 53
- Database :
- MEDLINE
- Journal :
- Human pathology
- Publication Type :
- Academic Journal
- Accession number :
- 27001433
- Full Text :
- https://doi.org/10.1016/j.humpath.2015.11.013