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Molecular cloning and biochemical characterization of Xaa-Pro dipeptidyl-peptidase from Streptococcus mutans and its inhibition by anti-human DPP IV drugs.
- Source :
-
FEMS microbiology letters [FEMS Microbiol Lett] 2016 May; Vol. 363 (9). Date of Electronic Publication: 2016 Mar 23. - Publication Year :
- 2016
-
Abstract
- Streptococcus mutans harbours an intracellular, human DPP IV-analogous enzyme Xaa-Pro dipeptidyl-peptidase (EC 3.4.14.11). According to previous reports, an extracellular isozyme in S. gordonii and S. suis has been associated with virulence. Speculating that even an intracellular form may aid in virulence of S. mutans, we have tried to purify, characterize and evaluate enzyme inhibition by specific inhibitors. The native enzyme was partially purified by ion-exchange and gel filtration chromatography. Owing to low yield, the enzyme was overexpressed in Lactococcus lactis and purified by affinity chromatography. The recombinant enzyme (rSm-XPDAP) had a specific activity of 1070 U mg(-1), while the Vmax and Km were 7 μM min(-1) and 89 ± 7 μM (n = 3), respectively. The serine protease inhibitor phenylmethylsulphonyl fluoride and a DPP IV-specific inhibitor diprotin A proved to be active against rSm-XPDAP. As a novel approach, the evaluation of the effect of anti-human DPP IV (AHD) drugs on rSm-XPDAP activity found saxagliptin to be effective to some extent (Ki = 129 ± 16 μM), which may lead to the synthesis and development of a new class of antimicrobial agents.<br /> (© FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Subjects :
- Adamantane analogs & derivatives
Adamantane pharmacology
Amino Acid Sequence
Chromatography, Gel
Cloning, Molecular
Dipeptides pharmacology
Dipeptidyl Peptidase 4 metabolism
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases chemistry
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases metabolism
Humans
Hydrogen-Ion Concentration
Kinetics
Lactococcus lactis genetics
Oligopeptides pharmacology
Recombinant Proteins chemistry
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Sequence Alignment
Streptococcus mutans drug effects
Substrate Specificity
Dipeptidyl-Peptidase IV Inhibitors pharmacology
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases antagonists & inhibitors
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases genetics
Streptococcus mutans enzymology
Streptococcus mutans genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1574-6968
- Volume :
- 363
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- FEMS microbiology letters
- Publication Type :
- Academic Journal
- Accession number :
- 27010012
- Full Text :
- https://doi.org/10.1093/femsle/fnw066