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Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics.

Authors :
Sarkar A
Hou HW
Mahan AE
Han J
Alter G
Source :
Scientific reports [Sci Rep] 2016 Mar 30; Vol. 6, pp. 23589. Date of Electronic Publication: 2016 Mar 30.
Publication Year :
2016

Abstract

Isolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required for many diagnostic, therapeutic and research applications. Current affinity-based protein or cell separation methods use binary 'bind-elute' separations and are inefficient when applied to the isolation of multiple low-abundance proteins or cell types. We present a method for rapid and multiplexed, yet inexpensive, affinity-based isolation of both proteins and cells, using a size-coded mixture of multiple affinity-capture microbeads and an inertial microfluidic particle sorter device. In a single binding step, different targets-cells or proteins-bind to beads of different sizes, which are then sorted by flowing them through a spiral microfluidic channel. This technique performs continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding. We demonstrate the simultaneous isolation of multiple antibodies from serum and multiple cell types from peripheral blood mononuclear cells or whole blood. We use the technique to isolate low abundance antibodies specific to different HIV antigens and rare HIV-specific cells from blood obtained from HIV+ patients.

Details

Language :
English
ISSN :
2045-2322
Volume :
6
Database :
MEDLINE
Journal :
Scientific reports
Publication Type :
Academic Journal
Accession number :
27026280
Full Text :
https://doi.org/10.1038/srep23589