Back to Search
Start Over
Monitoring Apoptosis of Breast Cancer Xenograft After Paclitaxel Treatment With 99mTc-Labeled Duramycin SPECT/CT.
- Source :
-
Molecular imaging [Mol Imaging] 2016 Jan 29; Vol. 15. Date of Electronic Publication: 2016 Jan 29 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- Our goal was to validate the feasibility of(99m)Tc-duramycin as a potential apoptosis probe for monitoring tumor response to paclitaxel in breast cancer xenografts. The binding of(99m)Tc-duramycin to phosphatidylethanolamine was validated in vitro using paclitaxel-treated human breast carcinoma MDA-MB-231 cells. Female BALB/c mice (n = 5) bearing breast cancer xenografts were randomized into 2 groups and intraperitoneally injected with 40 mg/kg paclitaxel or phosphate-buffered saline.(99m)Tc-duramycin (37-55.5 MBq) was injected at 72 hours posttreatment, and single-photon emission computed tomography/computed tomography was performed at 2 hours postinjection. Apoptotic cells and activated caspase 3 in explanted tumor tissue were measured by flow cytometry. Cellular ultrastructural changes were assessed by light and transmission electron microscopy.(99m)Tc-duramycin with radiochemical purity of >90% exhibited rapid blood clearance and predominantly renal clearance. The tumor-to-muscle ratio in the paclitaxel-treated group (5.29 ± 0.62) was significantly higher than that in the control. Tumor volume was decreased dramatically, whereas tumor uptake of(99m)Tc-duramycin (ex vivo) significantly increased following paclitaxel treatment, which was consistent with apoptotic index, histological findings, and ultrastructural changes. Our data demonstrated the feasibility of(99m)Tc-duramycin for early detection of apoptosis after paclitaxel chemotherapy in breast carcinoma xenografts.<br /> (© The Author(s) 2016.)
- Subjects :
- Animals
Antineoplastic Agents, Phytogenic pharmacology
Apoptosis
Bacteriocins pharmacokinetics
Breast Neoplasms drug therapy
Breast Neoplasms metabolism
Cell Line, Tumor
Feasibility Studies
Female
Humans
Mice
Organotechnetium Compounds pharmacokinetics
Paclitaxel pharmacology
Phosphatidylethanolamines metabolism
Radiopharmaceuticals pharmacokinetics
Random Allocation
Tomography, Emission-Computed, Single-Photon methods
Treatment Outcome
Xenograft Model Antitumor Assays
Antineoplastic Agents, Phytogenic administration & dosage
Bacteriocins administration & dosage
Breast Neoplasms diagnostic imaging
Organotechnetium Compounds administration & dosage
Paclitaxel administration & dosage
Radiopharmaceuticals administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1536-0121
- Volume :
- 15
- Database :
- MEDLINE
- Journal :
- Molecular imaging
- Publication Type :
- Academic Journal
- Accession number :
- 27030401
- Full Text :
- https://doi.org/10.1177/1536012115624918