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lncRHOXF1, a Long Noncoding RNA from the X Chromosome That Suppresses Viral Response Genes during Development of the Early Human Placenta.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2016 May 31; Vol. 36 (12), pp. 1764-75. Date of Electronic Publication: 2016 May 31 (Print Publication: 2016). - Publication Year :
- 2016
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Abstract
- Long noncoding RNAs (lncRNAs) can regulate gene expression in a cell-specific fashion during development. Here, we identify a novel lncRNA from the X chromosome that we named lncRHOXF1 and which is abundantly expressed in trophectoderm and primitive endoderm cells of human blastocyst-stage embryos. lncRHOXF1 is a spliced and polyadenylated lncRNA about 1 kb in length that is found in both the nuclear and cytoplasmic compartments of in vitro differentiated human trophectoderm progenitor cells. Gain-of-function experiments in human embryonic stem cells, which normally lack lncRHOXF1 RNA, revealed that lncRHOXF1 reduced proliferation and favored cellular differentiation. lncRHOXF1 knockdown using small interfering RNAs (siRNAs) in human trophectoderm progenitors increased expression of viral response genes, including type I interferon. Sendai virus infection of human trophectoderm progenitor cells increased lncRHOXF1 RNA levels, and siRNA-mediated disruption of lncRHOXF1 during infection reduced the expression of viral response genes leading to higher virus replication. Thus, lncRHOXF1 RNA is the first example of a lncRNA that regulates the host response to viral infections in human placental progenitor cells, and we propose that it functions as a repressor of the viral response during early human development.<br /> (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Animals
Cell Differentiation
Cell Nucleus genetics
Cell Proliferation
Cytoplasm genetics
Female
Humans
Mice
Polyadenylation
Pregnancy
Sendai virus physiology
Trophoblasts cytology
Trophoblasts immunology
Trophoblasts virology
Chromosomes, Human genetics
Human Embryonic Stem Cells cytology
Interferon Type I metabolism
Placentation
RNA, Long Noncoding genetics
Respirovirus Infections immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5549
- Volume :
- 36
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 27066803
- Full Text :
- https://doi.org/10.1128/MCB.01098-15