Back to Search
Start Over
Consolidated bioprocessing of poly(lactate-co-3-hydroxybutyrate) from xylan as a sole feedstock by genetically-engineered Escherichia coli.
Consolidated bioprocessing of poly(lactate-co-3-hydroxybutyrate) from xylan as a sole feedstock by genetically-engineered Escherichia coli.
- Source :
-
Journal of bioscience and bioengineering [J Biosci Bioeng] 2016 Oct; Vol. 122 (4), pp. 406-14. Date of Electronic Publication: 2016 Apr 08. - Publication Year :
- 2016
-
Abstract
- Consolidated bioprocessing of lignocellulose is an attractive strategy for the sustainable production of petroleum-based alternatives. One of the underutilized sources of carbon in lignocellulose is the hemicellulosic fraction which largely consists of the polysaccharide xylan. In this study, Escherichia coli JW0885 (pyruvate formate lyase activator protein mutant, pflA(-)) was engineered to express recombinant xylanases and polyhydroxyalkanoate (PHA)-producing enzymes for the biosynthesis of poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)] from xylan as a consolidated bioprocess. The results show that E. coli JW0885 was capable of producing P(LA-co-3HB) when xylan was the only feedstock and different feeding and growth parameters were examined in order to improve upon initial yields. The highest yields of P(LA-co-3HB) copolymer obtained in this study occurred when xylan was added during mid-exponential growth after cells had been grown at high shaking-speeds (290 rpm). The results showed an inverse relationship between total PHA production and LA-monomer incorporation into the copolymer. Proton nuclear magnetic resonance ((1)H NMR), gel permeation chromatography (GPC), and differential scanning calorimetry (DSC) analyses corroborate that the polymers produced maintain physical properties characteristic of LA-incorporating PHB-based copolymers. The present study achieves the first ever engineering of a consolidated bioprocessing bacterial system for the production of a bioplastic from a hemicelluosic feedstock.<br /> (Copyright © 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Biopolymers biosynthesis
Biopolymers metabolism
Calorimetry, Differential Scanning
Chromatography, Gel
Endo-1,4-beta Xylanases metabolism
Escherichia coli drug effects
Escherichia coli growth & development
Escherichia coli Proteins genetics
Polyhydroxyalkanoates biosynthesis
Polyhydroxyalkanoates metabolism
Polysaccharides chemistry
Polysaccharides metabolism
Proton Magnetic Resonance Spectroscopy
Xylans pharmacology
Escherichia coli genetics
Escherichia coli metabolism
Genetic Engineering
Polyesters metabolism
Xylans metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1347-4421
- Volume :
- 122
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of bioscience and bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 27067372
- Full Text :
- https://doi.org/10.1016/j.jbiosc.2016.03.009