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Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation.

Authors :
Lopandic K
Pfliegler WP
Tiefenbrunner W
Gangl H
Sipiczki M
Sterflinger K
Source :
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2016 Jul; Vol. 100 (14), pp. 6331-6343. Date of Electronic Publication: 2016 Apr 13.
Publication Year :
2016

Abstract

The yeasts of the Saccharomyces genus exhibit a low pre-zygotic barrier and readily form interspecies hybrids. Following the hybridization event, the parental genomes undergo gross chromosomal rearrangements and genome modifications that may markedly influence the metabolic activity of descendants. In the present study, two artificially constructed hybrid yeasts (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces kudriavzevii) were used in order to evaluate the influence of high-sugar wine fermentation on the evolution of their genotypic and phenotypic properties. It was demonstrated that the extent of genomic modifications differs among the hybrids and their progeny, but that stress should not always be a generator of large genomic disturbances. The major genome changes were observed after meiosis in the F1 segregants in the form of the loss of different non-S. cerevisiae chromosomes. Under fermentation condition, each spore clone from a tetrad developed a mixed population characterized by different genotypic and phenotypic properties. The S. cerevisiae x S. uvarum spore clones revealed large modifications at the sequence level of the S. cerevisiae sub-genome, and some of the clones lost a few additional S. cerevisiae and S. uvarum chromosomes. The S. cerevisiae x S. kudriavzevii segregants were subjected to consecutive loss of the S. kudriavzevii markers and chromosomes. Both the hybrid types showed increased ethanol and glycerol production as well as better sugar consumption than their parental strains. The hybrid segregants responded differently to stress and a correlation was found between the observed genotypes and fermentation performances.

Details

Language :
English
ISSN :
1432-0614
Volume :
100
Issue :
14
Database :
MEDLINE
Journal :
Applied microbiology and biotechnology
Publication Type :
Academic Journal
Accession number :
27075738
Full Text :
https://doi.org/10.1007/s00253-016-7481-0