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Comparison of Matrix-Based and Filter Paper-Based Systems for Transport of Plasma for HIV-1 RNA Quantification and Amplicon Preparation for Genotyping.
- Source :
-
Journal of clinical microbiology [J Clin Microbiol] 2016 Jul; Vol. 54 (7), pp. 1899-1901. Date of Electronic Publication: 2016 Apr 13. - Publication Year :
- 2016
-
Abstract
- Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensitivity of genotyping specimens with HIV-1 RNA of <4.70 log10 copies/ml.<br /> (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
Details
- Language :
- English
- ISSN :
- 1098-660X
- Volume :
- 54
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of clinical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 27076660
- Full Text :
- https://doi.org/10.1128/JCM.00541-16