Comparison of Matrix-Based and Filter Paper-Based Systems for Transport of Plasma for HIV-1 RNA Quantification and Amplicon Preparation for Genotyping.

Authors :: Levine M
Beck I
Styrchak S
Pepper G
Frenkel L
Source :: Journal of clinical microbiology [J Clin Microbiol] 2016 Jul; Vol. 54 (7), pp. 1899-1901. Date of Electronic Publication: 2016 Apr 13.
Publication Year :: 2016
Abstract: Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensitivity of genotyping specimens with HIV-1 RNA of <4.70 log10 copies/ml.<br /> (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)

Subjects :: HIV-1 genetics
Humans
Plasma virology
RNA, Viral genetics
Sensitivity and Specificity
Genotyping Techniques methods
HIV Infections virology
HIV-1 classification
HIV-1 isolation & purification
RNA, Viral isolation & purification
Specimen Handling methods
Viral Load methods

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