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Recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells.
- Source :
-
Vaccine [Vaccine] 2016 May 11; Vol. 34 (22), pp. 2490-5. Date of Electronic Publication: 2016 Apr 11. - Publication Year :
- 2016
-
Abstract
- Interleukin 7 (IL-7) has an important function in the development and maintenance of IL-17A+ γδ T cells. We here constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing antigen 85B (Ag85B)-IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG-Ag85B-IL-7. rBCG-Ag85B-IL-7 was the same in number as control rBCG expressing Ag85B (rBCG-Ag85B) in the lung at the early stage after intravenous inoculation, whereas the numbers of IL-17A+ γδ T cells and Ag-specific Th1 cells were significantly higher in the lungs of mice inoculated with rBCG-Ag85B-IL-7 than those inoculated with rBCG-Ag85B. The Ag-specific Th1 cell response was impaired in mice lacking IL-17A+ γδ T cells after inoculation with rBCG-Ag85B-IL-7. Thus, rBCG-Ag85B-IL-7 increases the pool size of IL-17A+ γδ T cells, which subsequently augment the Th1 response to mycobacterial infection.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
BCG Vaccine genetics
BCG Vaccine immunology
Cytokines immunology
Immunity, Innate
Interleukin-7 immunology
Lung immunology
Lung microbiology
Mice
Mice, Inbred C57BL
Mycobacterium bovis growth & development
Mycobacterium bovis immunology
Receptors, Antigen, T-Cell, gamma-delta immunology
Recombinant Fusion Proteins immunology
Th1 Cells immunology
Acyltransferases genetics
Acyltransferases immunology
Antigens, Bacterial genetics
Antigens, Bacterial immunology
Bacterial Proteins genetics
Bacterial Proteins immunology
Interleukin-17 immunology
Interleukin-7 genetics
Mycobacterium bovis genetics
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 34
- Issue :
- 22
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 27079930
- Full Text :
- https://doi.org/10.1016/j.vaccine.2016.03.096