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Enhancement of ganoderic acid production by constitutively expressing Vitreoscilla hemoglobin gene in Ganoderma lucidum.
- Source :
-
Journal of biotechnology [J Biotechnol] 2016 Jun 10; Vol. 227, pp. 35-40. Date of Electronic Publication: 2016 Apr 11. - Publication Year :
- 2016
-
Abstract
- The Vitreoscilla hemoglobin (VHb) gene was expressed in Ganoderma lucidum to enhance antitumor ganoderic acid (GA) production. The effects of VHb expression on the accumulation of GAs and lanosterol (intermediate) and the transcription of GA biosynthesis genes were also investigated. In VHb-expressing G. lucidum, the maximum concentrations of four individual GAs (GA-S, GA-T, GA-Mk and GA-Me) were 19.1±1.8, 34.6±2.1, 191.5±13.1 and 45.2±2.8μg/100mg dry weight, respectively, which were 1.4-, 2.2, 1.9- and 2.0-fold higher than those obtained in the wild-type strain. Moreover, the maximum lanosterol concentration in the strain expressing VHb was 1.28-fold lower than that in the wild-type strain. The transcription levels of 3-hydroxy-3-methylglutaryl coenzyme A reductase, squalene synthase, and lanosterol synthase genes were up-regulated by 1.6-, 1.5-, and 1.6-fold, respectively, in the strain expressing VHb. This work is beneficial in developing an efficient fermentation process for the hyperproduction of GAs.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Bacterial Proteins metabolism
Biomass
Biosynthetic Pathways genetics
Lanosterol metabolism
Time Factors
Transcription, Genetic
Transformation, Genetic
Truncated Hemoglobins metabolism
Bacterial Proteins genetics
Biotechnology methods
Gene Expression
Reishi genetics
Triterpenes metabolism
Truncated Hemoglobins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4863
- Volume :
- 227
- Database :
- MEDLINE
- Journal :
- Journal of biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 27080449
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2016.04.017