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Pittosporum cryptic virus 1: genome sequence completion using next-generation sequencing.
- Source :
-
Archives of virology [Arch Virol] 2016 Jul; Vol. 161 (7), pp. 2039-42. Date of Electronic Publication: 2016 Apr 18. - Publication Year :
- 2016
-
Abstract
- Next-generation sequencing (NGS) was applied to dsRNAs extracted from an Italian pittosporum plant infected with pittosporum cryptic virus 1 (PiCV1). NGS allowed assembly of the full genome sequence of PiCV1, comprising dsRNA1 (1.9 kbp) and dsRNA2 (1.5 kbp), which encode the RNA-dependent RNA polymerase and capsid protein genes, respectively. Phylogenetic and sequence analyses confirmed that PiCV1 is a new member of the genus Deltapartitivirus, family Partiviridae. From the same plant, NSG also permitted assembly of the complete genome sequence of eggplant mottled dwarf virus (EMDV), which shared 86 % to 98 % nucleotide sequence identity with complete and partial sequences (ca 6750 nt) of other known EMDV isolates with sequences available in the GenBank database.
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 161
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 27087112
- Full Text :
- https://doi.org/10.1007/s00705-016-2860-5