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Immune cells and type 1 IFN in urine of SLE patients correlate with immunopathology in the kidney.
- Source :
-
Clinical immunology (Orlando, Fla.) [Clin Immunol] 2016 Jul; Vol. 168, pp. 16-24. Date of Electronic Publication: 2016 Apr 18. - Publication Year :
- 2016
-
Abstract
- The immunopathological events in the kidneys of lupus nephritis (LN) patients are poorly understood due in part to the difficulty in acquiring serial biopsies and the inherent limitations in their analysis. To identify a means to circumvent these limitations, we investigated whether immune cells of kidney origin are present in patient urine and whether they correlate with kidney pathology. Flow cytometry analysis was performed on peripheral blood and urine cells of 69 SLE patients, of whom 41 were LN patients. In addition, type I IFN (IFNα/β) levels were determined in plasma and urine by bioassay. Approximately 60% of non-LN patients had urine lymphocytes. In these patients, T cells were always present and predominantly CD8(+), while B cells were either absent or a mixture of naïve and memory B cells. In contrast, >90% of LN patients had urine lymphocytes. In half, the B and T cells resembled those in non-LN patient urine; however, in the remaining patients, the B cells were exclusively Ig-secreting plasmablasts or plasma cells (PB/PCs) and the T cells were predominantly CD4(+). In addition, pDCs and IFNα/β frequently accompanied PB/PCs. The majority of patients with urine PB/PCs presented with proliferative nephritis and a significant loss of kidney function, which in some cases had progressed to end stage renal disease (ESRD). In conclusion, urine can provide access to cells of kidney resident populations for phenotypic and functional characterization. Analysis of these cells provides insight into the kidney immunopathology and may serve as biomarkers to identify patients at risk for developing LN and progressing to ESRD.<br /> (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Subjects :
- Adult
B-Lymphocytes immunology
CD8-Positive T-Lymphocytes immunology
Cell Line
Dendritic Cells immunology
Female
Flow Cytometry
Humans
Immune System immunology
Immunologic Memory immunology
Interferon Type I blood
Interferon Type I urine
Interferon-alpha blood
Interferon-alpha immunology
Interferon-alpha urine
Interferon-beta blood
Interferon-beta immunology
Interferon-beta urine
Kidney pathology
Kidney Failure, Chronic blood
Kidney Failure, Chronic immunology
Kidney Failure, Chronic urine
Lupus Erythematosus, Systemic blood
Lupus Erythematosus, Systemic urine
Lupus Nephritis blood
Lupus Nephritis urine
Male
Plasma Cells immunology
Immune System cytology
Interferon Type I immunology
Kidney immunology
Lupus Erythematosus, Systemic immunology
Lupus Nephritis immunology
Urine cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1521-7035
- Volume :
- 168
- Database :
- MEDLINE
- Journal :
- Clinical immunology (Orlando, Fla.)
- Publication Type :
- Academic Journal
- Accession number :
- 27102764
- Full Text :
- https://doi.org/10.1016/j.clim.2016.04.005