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Glycoengineered Outer Membrane Vesicles: A Novel Platform for Bacterial Vaccines.
- Source :
-
Scientific reports [Sci Rep] 2016 Apr 22; Vol. 6, pp. 24931. Date of Electronic Publication: 2016 Apr 22. - Publication Year :
- 2016
-
Abstract
- The World Health Organization has indicated that we are entering into a post-antibiotic era in which infections that were routinely and successfully treated with antibiotics can now be lethal due to the global dissemination of multidrug resistant strains. Conjugate vaccines are an effective way to create a long-lasting immune response against bacteria. However, these vaccines present many drawbacks such as slow development, high price, and batch-to-batch inconsistencies. Alternate approaches for vaccine development are urgently needed. Here we present a new vaccine consisting of glycoengineered outer membrane vesicles (geOMVs). This platform exploits the fact that the initial steps in the biosynthesis of most bacterial glycans are similar. Therefore, it is possible to easily engineer non-pathogenic Escherichia coli lab strains to produce geOMVs displaying the glycan of the pathogen of interest. In this work we demonstrate the versatility of this platform by showing the efficacy of geOMVs as vaccines against Streptococcus pneumoniae in mice, and against Campylobacter jejuni in chicken. This cost-effective platform could be employed to generate vaccines to prevent infections caused by a wide variety of microbial agents in human and animals.
- Subjects :
- Animals
Chickens
Mice
Pneumococcal Vaccines administration & dosage
Pneumococcal Vaccines isolation & purification
Vaccines, Conjugate administration & dosage
Vaccines, Conjugate immunology
Vaccines, Conjugate isolation & purification
Campylobacter jejuni immunology
Extracellular Vesicles immunology
Pneumococcal Vaccines immunology
Polysaccharides immunology
Streptococcus pneumoniae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27103188
- Full Text :
- https://doi.org/10.1038/srep24931