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Antiviral Role of IFITM Proteins in African Swine Fever Virus Infection.
- Source :
-
PloS one [PLoS One] 2016 Apr 26; Vol. 11 (4), pp. e0154366. Date of Electronic Publication: 2016 Apr 26 (Print Publication: 2016). - Publication Year :
- 2016
-
Abstract
- The interferon-induced transmembrane (IFITM) protein family is a group of antiviral restriction factors that impair flexibility and inhibit membrane fusion at the plasma or the endosomal membrane, restricting viral progression at entry. While IFITMs are widely known to inhibit several single-stranded RNA viruses, there are limited reports available regarding their effect in double-stranded DNA viruses. In this work, we have analyzed a possible antiviral function of IFITMs against a double stranded DNA virus, the African swine fever virus (ASFV). Infection with cell-adapted ASFV isolate Ba71V is IFN sensitive and it induces IFITMs expression. Interestingly, high levels of IFITMs caused a collapse of the endosomal pathway to the perinuclear area. Given that ASFV entry is strongly dependent on endocytosis, we investigated whether IFITM expression could impair viral infection. Expression of IFITM1, 2 and 3 reduced virus infectivity in Vero cells, with IFITM2 and IFITM3 having an impact on viral entry/uncoating. The role of IFITM2 in the inhibition of ASFV in Vero cells could be related to impaired endocytosis-mediated viral entry and alterations in the cholesterol efflux, suggesting that IFITM2 is acting at the late endosome, preventing the decapsidation stage of ASFV.
- Subjects :
- Animals
Cell Membrane metabolism
Chlorocebus aethiops
Cholesterol metabolism
Endocytosis
Endosomes metabolism
HEK293 Cells
Humans
Microscopy, Fluorescence
RNA Viruses metabolism
RNA, Double-Stranded
Vero Cells
African Swine Fever Virus pathogenicity
Antigens, Differentiation metabolism
Interferons metabolism
Membrane Proteins metabolism
Virus Internalization
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 11
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 27116236
- Full Text :
- https://doi.org/10.1371/journal.pone.0154366