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Bioinformatic Analysis of Potential microRNAs in Ischemic Stroke.
- Source :
-
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association [J Stroke Cerebrovasc Dis] 2016 Jul; Vol. 25 (7), pp. 1753-1759. Date of Electronic Publication: 2016 Apr 14. - Publication Year :
- 2016
-
Abstract
- Background: MicroRNAs (miRNAs) are part of the brain's response to ischemia. This study aimed to screen potential miRNAs for the prediction and novel treatments of ischemic stroke.<br />Methods: Two mRNA and 1 miRNA microarray expression profile data were downloaded from the Gene Expression Omnibus database. Then, differentially expressed mRNAs and miRNAs were identified. Based on the miRNA-target pairs predicted, an miRNA-target interaction network was established. Bioinformatics analysis (Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment) was applied to interpret the function of the miRNA targets.<br />Results: In total, 16 differentially expressed miRNAs and 1345 differentially expressed genes were identified in ischemic stroke, respectively. Importantly, 445 miRNA-target pairs with an inverse correlation of expression were obtained, and the miRNA functional synergistic network was generated. Two miRNAs (miR-145 and miR-122) may represent potential biomarkers in ischemic stroke by being involved in the process of postischemic neuronal damage and thrombosis, respectively. Three novel miRNAs (miR-99b, miR-542-3p, and miR-455-5p) were deregulated, suggesting their roles in the pathological processes of ischemic stroke. Functional annotation indicated that apoptotic signaling cascades play an important role in patients with ischemic stroke.<br />Conclusion: miR-145 and miR-122 represent new biomarkers and underlying targets for the prevention and treatment of ischemic stroke.<br /> (Copyright © 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Brain Ischemia diagnosis
Case-Control Studies
Databases, Genetic
Gene Expression Profiling methods
Gene Expression Regulation
Gene Regulatory Networks
Genetic Association Studies
Genetic Markers
Genetic Predisposition to Disease
Humans
Oligonucleotide Array Sequence Analysis
Phenotype
Stroke diagnosis
Brain Ischemia genetics
Computational Biology
MicroRNAs genetics
Stroke genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1532-8511
- Volume :
- 25
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association
- Publication Type :
- Academic Journal
- Accession number :
- 27151415
- Full Text :
- https://doi.org/10.1016/j.jstrokecerebrovasdis.2016.03.023