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Roles of interferon-stimulated gene 15 protein in bovine embryo development.
- Source :
-
Reproduction, fertility, and development [Reprod Fertil Dev] 2017 Jun; Vol. 29 (6), pp. 1209-1216. - Publication Year :
- 2017
-
Abstract
- Interferon (IFN)-stimulated gene 15 (ISG15) is one of several proteins induced by conceptus-derived Type I or II IFNs in the uterus, and is implicated as an important factor in determining uterine receptivity to embryos in ruminants. But little is known about the role the ISG15 gene or gene product plays during embryo development. In the present study, both the expression profile and function of ISG15 were investigated in early bovine embryos in vitro. ISG15 mRNA was detectable in Day 0, 2, 6 and 8 bovine embryos, but IFN-τ (IFNT) mRNA only appeared from Day 6. This means that embryonic expression of ISG15 on Days 0 and 2 was not induced by embryonic IFNT. However, ISG15 mRNA expression paralleled the expression of IFNT mRNA in Day 6 and 8 embryos. ISG15-lentivirus interference plasmid (ISG15i) was injected into 2-cell embryos to knockdown ISG15 expression. This resulted in decreases in the proportion of hatching blastocysts, the diameter of blastocysts and cell number per diameter of blastocysts compared with control embryos. In addition, ISG15i inhibited IFNT, Ets2 (E26 oncogene homolog 2) mRNA and connexion 43 protein expression in Day 8 blastocysts, whereas exogenous IFNT treatment (100ngmL <superscript>-1</superscript> , from Day 4 to Day 8) improved ISG15 mRNA and connexion 43 protein expression. In conclusion, it appears that ISG15 is involved in early bovine embryo development and that it regulates IFNT expression in the blastocyst.
- Subjects :
- Abattoirs
Animals
Animals, Inbred Strains
Blastocyst cytology
Blastocyst drug effects
Cattle
Cryopreservation
Cytokines antagonists & inhibitors
Cytokines genetics
Female
Fertilization in Vitro
Gene Expression Profiling
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
In Vitro Oocyte Maturation Techniques
Interferon Type I genetics
Interferon Type I pharmacology
Male
Pregnancy Proteins genetics
Pregnancy Proteins pharmacology
RNA Interference
Recombinant Fusion Proteins metabolism
Recombinant Proteins metabolism
Recombinant Proteins pharmacology
Semen Preservation
Ubiquitins antagonists & inhibitors
Ubiquitins genetics
Blastocyst metabolism
Cytokines metabolism
Ectogenesis drug effects
Gene Expression Regulation, Developmental drug effects
Interferon Type I metabolism
Pregnancy Proteins metabolism
Ubiquitins metabolism
Up-Regulation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1031-3613
- Volume :
- 29
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Reproduction, fertility, and development
- Publication Type :
- Academic Journal
- Accession number :
- 27165775
- Full Text :
- https://doi.org/10.1071/RD15209