Back to Search Start Over

Magnetic Nanoparticles as a Potential Vehicle for Corneal Endothelium Repair.

Authors :
Cornell LE
Wehmeyer JL
Johnson AJ
Desilva MN
Zamora DO
Source :
Military medicine [Mil Med] 2016 May; Vol. 181 (5 Suppl), pp. 232-9.
Publication Year :
2016

Abstract

The corneal endothelium is paramount to the health and function of the cornea as damage to this cell layer can lead to corneal edema, opacification, and ultimately vision loss. Transplantation of the corneal endothelium is associated with numerous limitations, including graft rejection, thus an alternative therapeutic treatment is needed to restore endothelial layer integrity. We hypothesize that a nanotechnology-based approach using superparamagnetic iron oxide nanoparticles (SPIONPs) can ultimately be used to guide corneal endothelial cells (CECs) to injured areas via an external magnetic force without changing their morphology or viability. In this feasibility study we examined the effects of SPIONPs on the morphology and viability of bovine CECs in the presence of a magnetic force. The CECs were exposed to increasing SPIONP concentrations and the viability and cytoskeletal structure assessed over 3 days via metabolic analysis and rhodamine phalloidin staining. Significant differences (p < .05) in the metabolic activity of the CECs (100 × 10(6) SPIONP/cell) occurred in the presence of magnetic force versus those with no magnetic force. No differences were observed in the cytoskeleton of CECs in the presence or absence of magnetic force for all SPIONP concentrations. These SPIONPs will next be evaluated with human CECs for future applications.<br /> (Reprint & Copyright © 2016 Association of Military Surgeons of the U.S.)

Details

Language :
English
ISSN :
1930-613X
Volume :
181
Issue :
5 Suppl
Database :
MEDLINE
Journal :
Military medicine
Publication Type :
Academic Journal
Accession number :
27168578
Full Text :
https://doi.org/10.7205/MILMED-D-15-00151