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Plasma metabolomics combined with lipidomics profiling reveals the potential antipyretic mechanisms of Qingkailing injection in a rat model.
- Source :
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Chemico-biological interactions [Chem Biol Interact] 2016 Jul 25; Vol. 254, pp. 24-33. Date of Electronic Publication: 2016 May 18. - Publication Year :
- 2016
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Abstract
- Qingkailing injection (QKLI) has a notable antipyretic effect and is widely used in China as a clinical emergency medicine. To elucidate the pharmacological action thoroughly, following the investigation of the urine metabolome and hypothalamus metabolome, plasma metabolomics combined with lipidomics profiling of the QKLI antipyretic effect in a rat model is described in this paper. Compared with pure metabolomics profiling, this non-targeted plasma metabolomics combined with lipidomics profiling based on ultra-performance liquid chromatography-coupled with quadrupole time-of-flight mass spectrometry (UPLC Q-TOF/MS) could be used for a large-scale detection of features in plasma samples. The results showed that 15 metabolites at the 1 h time point and 19 metabolites at the 2 h time point after QKLI administration were associated with the antipyretic effect of QKLI, including amino acid, phosphatidylcholine and lysophosphatidylcholine. The metabolism pathway analysis revealed that the potential biomarkers, which were important for the antipyretic mechanism of QKLI, were closely responsible for correcting the perturbed pathways of amino acid metabolism and lipid metabolism. In conclusion, the use of complementary UPLC Q-TOF/MS based metabolomics and lipidomics allows for the discovery of new potential plasma biomarkers in the QKLI antipyretic process and the associated pathways, and aided in advancing the understanding of the holism and synergism of the Chinese drug.<br /> (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Animals
Antipyretics analysis
Antipyretics pharmacology
Biomarkers blood
Body Temperature drug effects
Chromatography, High Pressure Liquid
Discriminant Analysis
Drugs, Chinese Herbal analysis
Drugs, Chinese Herbal pharmacology
Least-Squares Analysis
Models, Animal
Rats
Spectrometry, Mass, Electrospray Ionization
Time Factors
Antipyretics metabolism
Drugs, Chinese Herbal metabolism
Lipids blood
Metabolomics
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7786
- Volume :
- 254
- Database :
- MEDLINE
- Journal :
- Chemico-biological interactions
- Publication Type :
- Academic Journal
- Accession number :
- 27208622
- Full Text :
- https://doi.org/10.1016/j.cbi.2016.05.022