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Simultaneous enantioselective quantification of fluoxetine and norfluoxetine in human milk by direct sample injection using 2-dimensional liquid chromatography-tandem mass spectrometry.

Authors :
Alvim J Jr
Lopes BR
Cass QB
Source :
Journal of chromatography. A [J Chromatogr A] 2016 Jun 17; Vol. 1451, pp. 120-126. Date of Electronic Publication: 2016 May 06.
Publication Year :
2016

Abstract

A two-dimensional liquid chromatography system coupled to triple quadrupole tandem mass spectrometer (2D LC-MS/MS) was employed for the simultaneously quantification of fluoxetine (FLX) and norfluoxetine (NFLX) enantiomers in human milk by direct injection of samples. A restricted access media of bovine serum albumin octadecyl column (RAM-BSAC18) was used in the first dimension for the milk proteins depletion, while an antibiotic-based chiral column was used in the second dimension. The results herein described show good selectivity, extraction efficiency, accuracy, and precision with limits of quantification in the order of 7.5ngmL(-1)for the FLX enantiomers and 10.0ngmL(-1) for NFLX enantiomers. Furthermore, it represents a practical tool in terms of sustainability for the sample preparation of such a difficult matrix.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3778
Volume :
1451
Database :
MEDLINE
Journal :
Journal of chromatography. A
Publication Type :
Academic Journal
Accession number :
27208983
Full Text :
https://doi.org/10.1016/j.chroma.2016.05.022