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MicroRNA-126 contributes to Niaspan treatment induced vascular restoration after diabetic retinopathy.
- Source :
-
Scientific reports [Sci Rep] 2016 May 26; Vol. 6, pp. 26909. Date of Electronic Publication: 2016 May 26. - Publication Year :
- 2016
-
Abstract
- Diabetic retinopathy (DR) is a serious microvascular complication of diabetes and a major cause of blindness in the developing world. Early diabetic retinopathy is characterized by a loss of pericytes and vascular endothelial cells, a breakdown of the blood-retinal barrier, vascular dysfunction and vascular-neuroinflammation. However, optimal treatment options and related mechanisms are still unclear. MicroRNA-126 (miR-126) plays a potential role in the pathogenesis in DR, which may regulate VEGF, Ang-1 and VCAM-1 expressions. This study investigated the therapeutic effects and mechanisms of Niaspan treatment of DR in diabetes (DM) rats. DM rats exhibits significantly decreased miR-126 and tight junction Claudin-5/Occludin/ZO-1 genes expression, and increased Blood retinal-barrier (BRB) breakdown, retinal apoptosis and VEGF/VEGFR, as well as VCAM-1/CD45 expressions in the retina compared to normal control group. Niaspan treatment significantly improved clinical and histopathological outcomes; decreased the expressions of VEGF/VEGFR, VCAM-1/CD45, apoptosis and BRB breakdown, significantly increased tight junction proteins and Ang-1/Tie-2 expressions, as well as increased retinal miR-126 expression compared to non-treatment diabetic rats. These data are the first to show that Niaspan treatment ameliorates DR through its repair vascular and inhibits inflammatory effects, and also suggest that the miR-126 pathway may contribute to Niaspan treatment induced benefit effects.
- Subjects :
- Angiogenic Proteins genetics
Angiogenic Proteins metabolism
Animals
Apoptosis
Ascorbic Acid
Blood Glucose
Cells, Cultured
Cholecalciferol
Cholesterol blood
Dehydroepiandrosterone analogs & derivatives
Delayed-Action Preparations
Diabetes Mellitus, Experimental blood
Diabetic Retinopathy blood
Diabetic Retinopathy genetics
Diabetic Retinopathy metabolism
Drug Evaluation, Preclinical
Gene Expression drug effects
Humans
Hypolipidemic Agents administration & dosage
Male
Nicotinic Acids
Plant Extracts
RNA Interference
Rats, Wistar
Retina drug effects
Retina metabolism
Retinal Vessels drug effects
Tight Junction Proteins metabolism
Tight Junctions metabolism
Angiogenesis Inducing Agents administration & dosage
Diabetes Mellitus, Experimental complications
Diabetic Retinopathy drug therapy
MicroRNAs physiology
Niacin administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27225425
- Full Text :
- https://doi.org/10.1038/srep26909