Molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis.

Authors :: Gou JB
Li ZQ
Li CF
Chen FF
Lv SY
Zhang YS
Source :: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2016 Sep; Vol. 106, pp. 288-94. Date of Electronic Publication: 2016 May 18.
Publication Year :: 2016
Abstract: Junenol based-eudesmanolides have been detected in many compositae plant species and were reported to exhibit various pharmacological activities. So far, the gene encoding junenol synthase has never been isolated. Here we report the molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis (designated IhsTPS1). IhsTPS1 converts the substrate farnesyl diphosphate into multiple sesquiterpenes with the product 10-epi-junenol being predominant. The transcript levels of IhsTPS1 correlate well with the accumulation pattern of 10-epi-junenol in I. hupehensis organs, supporting its biochemical roles in vivo.<br /> (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)

Subjects :: Alkyl and Aryl Transferases metabolism
Biosynthetic Pathways genetics
Cloning, Molecular
DNA, Complementary genetics
Gas Chromatography-Mass Spectrometry
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Phylogeny
Plant Proteins metabolism
Polyisoprenyl Phosphates metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Sesquiterpenes chemistry
Sesquiterpenes metabolism
Alkyl and Aryl Transferases genetics
Inula enzymology
Inula genetics
Plant Proteins genetics

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