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Arachidonic acid metabolomic study of BPH in rats and the interventional effects of Zishen pill, a traditional Chinese medicine.
- Source :
-
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2016 Sep 05; Vol. 128, pp. 149-157. Date of Electronic Publication: 2016 May 17. - Publication Year :
- 2016
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Abstract
- Zishen pill (ZSP) is a traditional Chinese medicine (TCM) used to treat benign prostatic hyperplasia (BPH). The study used a metabolomic approach based on UHPLC-MS/MS to profile arachidonic acid (AA) metabolic changes and to investigate the interventional mechanisms of ZSP in testosterone- induced BPH rats. In order to explore the potential therapeutic effect of ZSP, rat models were constructed and orally administrated with ZSP. Plasma and urine samples were collected after four weeks and then eleven potential biomarkers (15-HETE, 12-HETE, TXA2, 5-HETE, AA, PGI2, PGF2α, 8-HETE, PGD2, PGE2 and LTB4) were identified and quantified by UHPLC-MS/MS. The chromatographic separation was carried out with gradient elution using a mobile phase comprised of 0.05% formic acid aqueous solution (pH=3.3) (A) and acetonitrile: methanol (80:20, V/V) (B), and each AA metabolites was measured using electrospray ionization source with negative mode and multiple reaction monitoring. The eleven biomarkers in BPH group rat plasma and urine were significant higher than those in sham group rats. Using the potential biomarkers as a screening index, the results suggest that ZSP can potentially reverse the process of BPH by partially regulating AA metabolism through refrain the expression of cyclooxygenase (COX) and lipoxygenase (LOX). This study demonstrates that a metabolomic strategy is useful for identifying potential BPH biomarkers and investigating the underlying mechanisms of a TCM in BPH treatment.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid metabolism
Animals
Biomarkers metabolism
Chromatography, High Pressure Liquid methods
Drugs, Chinese Herbal metabolism
Hydroxyeicosatetraenoic Acids metabolism
Lipoxygenase metabolism
Male
Medicine, Chinese Traditional methods
Metabolomics methods
Plasma metabolism
Prostaglandin-Endoperoxide Synthases metabolism
Rats
Rats, Sprague-Dawley
Tandem Mass Spectrometry methods
Arachidonic Acid metabolism
Prostatic Hyperplasia metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-264X
- Volume :
- 128
- Database :
- MEDLINE
- Journal :
- Journal of pharmaceutical and biomedical analysis
- Publication Type :
- Academic Journal
- Accession number :
- 27262108
- Full Text :
- https://doi.org/10.1016/j.jpba.2016.05.027