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IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy.
- Source :
-
Scientific reports [Sci Rep] 2016 Jun 06; Vol. 6, pp. 27149. Date of Electronic Publication: 2016 Jun 06. - Publication Year :
- 2016
-
Abstract
- Increasingly, inflammatory mediators are considered crucial to the onset and perpetuation of tendinopathy. We sought evidence of interleukin 17A (IL-17A) expression in early human tendinopathy and thereafter, explored mechanisms whereby IL-17A mediated inflammation and tissue remodeling in human tenocytes. Torn supraspinatus tendon (established pathology) and matched intact subscapularis tendon (representing 'early pathology') along with control biopsies were collected from patients undergoing shoulder surgery. Markers of inflammation and IL-17A were quantified by RT-PCR and immunohistochemistry. Human tendon cells were derived from hamstring tendon obtained during ACL reconstruction. In vitro effects of IL-17A upon tenocytes were measured using RT-PCR, multiplex cytokine assays, apoptotic proteomic profiling, immunohistochemistry and annexin V FACS staining. Increased expression of IL-17A was detected in 'early tendinopathy' compared to both matched samples and non-matched control samples (p < 0.01) by RT-PCR and immunostaining. Double immunofluoresence staining revealed IL-17A expression in leukocyte subsets including mast cells, macrophages and T cells. IL-17A treated tenocytes exhibited increased production of proinflammatory cytokines (p < 0.001), altered matrix regulation (p < 0.01) with increased Collagen type III and increased expression of several apoptosis related factors. We propose IL-17A as an inflammatory mediator within the early tendinopathy processes thus providing novel therapeutic approaches in the management of tendon disorders.
- Subjects :
- Adolescent
Adult
Aged
Apoptosis
Cytokines genetics
Cytokines metabolism
Female
Humans
Leukocytes metabolism
Male
Middle Aged
Proteomics
Rotator Cuff Injuries genetics
Rotator Cuff Injuries metabolism
Rotator Cuff Injuries surgery
Tendinopathy genetics
Tendinopathy metabolism
Tendinopathy surgery
Tenocytes cytology
Tenocytes metabolism
Up-Regulation
Young Adult
Interleukin-17 genetics
Interleukin-17 metabolism
Rotator Cuff Injuries pathology
Tendinopathy pathology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27263531
- Full Text :
- https://doi.org/10.1038/srep27149