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An HBV-tolerant immunocompetent model that effectively simulates chronic hepatitis B virus infection in mice.
- Source :
-
Experimental animals [Exp Anim] 2016 Nov 01; Vol. 65 (4), pp. 373-382. Date of Electronic Publication: 2016 Jun 06. - Publication Year :
- 2016
-
Abstract
- Hepatitis B virus (HBV) is the leading cause of liver disease and hepatic carcinoma (HCC). Approximately 350 million people worldwide are infected with HBV and at risk of chronicity. An efficient HBV-tolerant murine model that mimics HBV infection in humans is desirable for HBV-related research. In this study, we investigated and established a murine model by hydrodynamic injection (HDI) of pAAV/HBV into the tail vein of AAVS1 site element-transgenic mice. In 80% of the injected mice, the serum level of HBsAg reached 10 <superscript>3-4</superscript> IU/ml and persisted for more than half a year. Next, the model was used to evaluate RNA interference (RNAi)-based antiviral therapy. Data obtained using the model demonstrated that this model will facilitate the elucidation of the mechanisms underlying chronic HBV infection and will also be useful for evaluating new antiviral drugs.
- Subjects :
- Animals
Antiviral Agents pharmacology
Disease Models, Animal
Female
Hepatitis B Surface Antigens blood
Hepatitis B, Chronic drug therapy
Hepatitis B, Chronic genetics
Humans
Hydrodynamics
Injections
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Transgenic
RNA Interference
Hepatitis B virus physiology
Hepatitis B, Chronic etiology
Subjects
Details
- Language :
- English
- ISSN :
- 1881-7122
- Volume :
- 65
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Experimental animals
- Publication Type :
- Academic Journal
- Accession number :
- 27264142
- Full Text :
- https://doi.org/10.1538/expanim.16-0013