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STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells.
- Source :
-
Scientific reports [Sci Rep] 2016 Jun 08; Vol. 6, pp. 26691. Date of Electronic Publication: 2016 Jun 08. - Publication Year :
- 2016
-
Abstract
- Stathmin 1 (STMN1) is a biomarker in several types of neoplasms. It plays an important role in cell cycle progression, mitosis, signal transduction and cell migration. In ovaries, STMN1 is predominantly expressed in granulosa cells (GCs). However, little is known about the role of STMN1 in ovary. In this study, we demonstrated that STMN1 is overexpressed in GCs in patients with polycystic ovary syndrome (PCOS). In mouse primary GCs, the overexpression of STMN1 stimulated progesterone production, whereas knockdown of STMN1 decreased progesterone production. We also found that STMN1 positively regulates the expression of Star (steroidogenic acute regulatory protein) and Cyp11a1 (cytochrome P450 family 11 subfamily A member 1). Promoter and ChIP assays indicated that STMN1 increased the transcriptional activity of Star and Cyp11a1 by binding to their promoter regions. The data suggest that STMN1 mediates the progesterone production by modulating the promoter activity of Star and Cyp11a1. Together, our findings provide novel insights into the molecular mechanisms of STMN1 in ovary GC steroidogenesis. A better understanding of this potential interaction between STMN1 and Star in progesterone biosynthesis in GCs will facilitate the discovery of new therapeutic targets in PCOS.
- Subjects :
- Animals
Cholesterol Side-Chain Cleavage Enzyme metabolism
Female
Granulosa Cells pathology
Humans
Macaca mulatta
Mice
Polycystic Ovary Syndrome metabolism
Polycystic Ovary Syndrome pathology
Granulosa Cells metabolism
Phosphoproteins biosynthesis
Progesterone biosynthesis
Stathmin metabolism
Up-Regulation
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27270953
- Full Text :
- https://doi.org/10.1038/srep26691