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CRACM3 regulates the stability of non-excitable exocytotic vesicle fusion pores in a Ca(2+)-independent manner via molecular interaction with syntaxin4.
- Source :
-
Scientific reports [Sci Rep] 2016 Jun 15; Vol. 6, pp. 28133. Date of Electronic Publication: 2016 Jun 15. - Publication Year :
- 2016
-
Abstract
- Ca(2+) release-activated calcium channel 3 (CRACM3) is a unique member of the CRAC family of Ca(2+)-selective channels. In a non-excitable exocytosis model, we found that the extracellular L3 domain and the cytoplasmic C-terminus of CRACM3 interacted in an activity-dependent manner with the N-peptide of syntaxin4, a soluble N-ethylmaleimide-sensitive factor attachment receptor protein. Our biochemical, electrophysiological and single-vesicle studies showed that knockdown of CRACM3 suppressed functional exocytosis by decreasing the open time of the vesicle fusion pore without affecting Ca(2+) influx, the activity-dependent membrane capacitance (Cm) change, and the total number of fusion events. Conversely, overexpressing CRACM3 significantly impaired cell exocytosis independent of Ca(2+), led to an impaired Cm change, decreased the number of fusion events, and prolonged the dwell time of the fusion pore. CRACM3 changes the stability of the vesicle fusion pore in a manner consistent with the altered molecular expression. Our findings imply that CRACM3 plays a greater role in exocytosis than simply acting as a compensatory subunit of a Ca(2+) channel.
- Subjects :
- Animals
Calcium Channels genetics
Calcium Release Activated Calcium Channels metabolism
Cell Line, Tumor
Exocytosis drug effects
Membrane Fusion physiology
Qa-SNARE Proteins genetics
Rats
Secretory Vesicles metabolism
Single-Cell Analysis
Thapsigargin pharmacology
Calcium metabolism
Calcium Channels physiology
Exocytosis physiology
Qa-SNARE Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 6
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 27301714
- Full Text :
- https://doi.org/10.1038/srep28133