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Plasmid-Borne Antimicrobial Resistance of Staphylococcus aureus Isolated in a Hospital in Lisbon, Portugal.
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2016 Dec; Vol. 22 (8), pp. 617-626. Date of Electronic Publication: 2016 Jun 16. - Publication Year :
- 2016
-
Abstract
- Plasmids play a key role in the genetic plasticity and survival of Staphylococcus aureus in challenging environments. Although many S. aureus plasmids have been described, still few studies portray the plasmid content of a given S. aureus population. The aim of this work was to characterize the plasmids carried by a collection of 53 S. aureus isolates collected in a large hospital in Lisbon, Portugal, and investigate their role in conferring resistance to several antimicrobial agents. Plasmids were present in 44 out of the 53 isolates and were grouped into eleven AccI restriction profiles. Plasmid curing of representative strains and comparison of antimicrobial susceptibility profiles between pairs of isogenic strains proved to be a valuable guidance tool in the identification of plasmid-located resistance genes. The plasmids harbored several resistance genes, namely blaZ (resistance to β-lactams), erm(C) (resistance to macrolides, lincosamides, and streptogramin B), cadA (resistance to cadmium and zinc), cadD (resistance to cadmium), and qacA and smr (resistance to biocides and dyes). This study demonstrates the impact of plasmids on the resistance properties of S. aureus, highlighting their role in the dissemination of antibiotic, heavy metal, and biocide resistance genes, and survival of this major pathogen in the hospital environment.
- Subjects :
- Adenosine Triphosphatases genetics
Adenosine Triphosphatases metabolism
Anti-Bacterial Agents pharmacology
Antiporters genetics
Antiporters metabolism
Bacterial Proteins genetics
Bacterial Proteins metabolism
Hospitals
Humans
Membrane Proteins genetics
Membrane Proteins metabolism
Membrane Transport Proteins genetics
Membrane Transport Proteins metabolism
Methyltransferases genetics
Methyltransferases metabolism
Microbial Sensitivity Tests
Multilocus Sequence Typing
Plasmids chemistry
Portugal
Staphylococcal Infections drug therapy
Staphylococcal Infections microbiology
Staphylococcus aureus drug effects
Staphylococcus aureus growth & development
Staphylococcus aureus isolation & purification
Drug Resistance, Multiple, Bacterial genetics
Gene Expression Regulation, Bacterial
Plasmids metabolism
Staphylococcus aureus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 22
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 27309487
- Full Text :
- https://doi.org/10.1089/mdr.2015.0352