Back to Search
Start Over
Reduced epithelial suppressor of cytokine signalling 1 in severe eosinophilic asthma.
- Source :
-
The European respiratory journal [Eur Respir J] 2016 Sep; Vol. 48 (3), pp. 715-25. Date of Electronic Publication: 2016 Jun 23. - Publication Year :
- 2016
-
Abstract
- Severe asthma represents a major unmet clinical need. Eosinophilic inflammation persists in the airways of many patients with uncontrolled asthma, despite high-dose inhaled corticosteroid therapy. Suppressors of cytokine signalling (SOCS) are a family of molecules involved in the regulation of cytokine signalling via inhibition of the Janus kinase-signal transducers and activators of transcription pathway. We examined SOCS expression in the airways of asthma patients and investigated whether this is associated with persistent eosinophilia.Healthy controls, mild/moderate asthmatics and severe asthmatics were studied. Whole genome expression profiling, quantitative PCR and immunohistochemical analysis were used to examine expression of SOCS1, SOCS2 and SOCS3 in bronchial biopsies. Bronchial epithelial cells were utilised to examine the role of SOCS1 in regulating interleukin (IL)-13 signalling in vitroSOCS1 gene expression was significantly lower in the airways of severe asthmatics compared with mild/moderate asthmatics, and was inversely associated with airway eosinophilia and other measures of T-helper type 2 (Th2) inflammation. Immunohistochemistry demonstrated SOCS1 was predominantly localised to the bronchial epithelium. SOCS1 overexpression inhibited IL-13-mediated chemokine ligand (CCL) 26 (eotaxin-3) mRNA expression in bronchial epithelial cells.Severe asthma patients with persistent airway eosinophilia and Th2 inflammation have reduced airway epithelial SOCS1 expression. SOCS1 inhibits epithelial IL-13 signalling, supporting its key role in regulating Th2-driven eosinophilia in severe asthma.<br /> (Copyright ©ERS 2016.)
- Subjects :
- Adult
Asthma drug therapy
Biopsy
Bronchi metabolism
Bronchoscopy
Case-Control Studies
Cell Line
Chemokine CCL26 metabolism
Cohort Studies
Female
Gene Expression Profiling
Humans
Inflammation
Male
Middle Aged
Pulmonary Eosinophilia drug therapy
Respiratory Mucosa metabolism
Signal Transduction
Suppressor of Cytokine Signaling 3 Protein metabolism
Suppressor of Cytokine Signaling Proteins metabolism
Th2 Cells cytology
Young Adult
Asthma metabolism
Epithelial Cells metabolism
Interleukin-13 metabolism
Pulmonary Eosinophilia metabolism
Suppressor of Cytokine Signaling 1 Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1399-3003
- Volume :
- 48
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The European respiratory journal
- Publication Type :
- Academic Journal
- Accession number :
- 27338192
- Full Text :
- https://doi.org/10.1183/13993003.00400-2015