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Enhancing hydrogen production of Enterobacter aerogenes by heterologous expression of hydrogenase genes originated from Synechocystis sp.
- Source :
-
Bioresource technology [Bioresour Technol] 2016 Sep; Vol. 216, pp. 976-80. Date of Electronic Publication: 2016 Jun 16. - Publication Year :
- 2016
-
Abstract
- The hydrogenase genes (hoxEFUYH) of Synechocystis sp. PCC 6803 were cloned and heterologously expressed in Enterobacter aerogenes ATCC13408 for the first time in this study, and the hydrogen yield was significantly enhanced using the recombinant strain. A recombinant plasmid containing the gene in-frame with Glutathione-S-Transferase (GST) gene was transformed into E. aerogenes ATCC13408 to produce a GST-fusion protein. SDS-PAGE and western blot analysis confirm the successful expression of the hox genes. The hydrogenase activity of the recombinant strain is 237.6±9.3ml/(g-DW·h), which is 152% higher than the wild strain. The hydrogen yield of the recombinant strain is 298.3ml/g-glucose, which is 88% higher than the wild strain. During hydrogen fermentation, the recombinant strain produces more acetate and butyrate, but less ethanol. This is corresponding to the NADH metabolism in the cell due to the higher hydrogenase activity with the heterologous expression of hox genes.<br /> (Copyright © 2016 Elsevier Ltd. All rights reserved.)
- Subjects :
- Fermentation
Bacterial Proteins genetics
Bacterial Proteins metabolism
Enterobacter aerogenes genetics
Enterobacter aerogenes metabolism
Hydrogen analysis
Hydrogen metabolism
Hydrogenase genetics
Hydrogenase metabolism
Recombinant Proteins genetics
Recombinant Proteins metabolism
Synechocystis enzymology
Synechocystis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 216
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 27343449
- Full Text :
- https://doi.org/10.1016/j.biortech.2016.06.044