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Fermentative lactic acid production from coffee pulp hydrolysate using Bacillus coagulans at laboratory and pilot scales.

Authors :
Pleissner D
Neu AK
Mehlmann K
Schneider R
Puerta-Quintero GI
Venus J
Source :
Bioresource technology [Bioresour Technol] 2016 Oct; Vol. 218, pp. 167-73. Date of Electronic Publication: 2016 Jun 21.
Publication Year :
2016

Abstract

In this study, the lignocellulosic residue coffee pulp was used as carbon source in fermentative l(+)-lactic acid production using Bacillus coagulans. After thermo-chemical treatment at 121°C for 30min in presence of 0.18molL(-1) H2SO4 and following an enzymatic digestion using Accellerase 1500 carbon-rich hydrolysates were obtained. Two different coffee pulp materials with comparable biomass composition were used, but sugar concentrations in hydrolysates showed variations. The primary sugars were (gL(-1)) glucose (20-30), xylose (15-25), sucrose (5-11) and arabinose (0.7-10). Fermentations were carried out at laboratory (2L) and pilot (50L) scales in presence of 10gL(-1) yeast extract. At pilot scale carbon utilization and lactic acid yield per gram of sugar consumed were 94.65% and 0.78gg(-1), respectively. The productivity was 4.02gL(-1)h(-1). Downstream processing resulted in a pure formulation containing 937gL(-1)l(+)-lactic acid with an optical purity of 99.7%.<br /> (Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Details

Language :
English
ISSN :
1873-2976
Volume :
218
Database :
MEDLINE
Journal :
Bioresource technology
Publication Type :
Academic Journal
Accession number :
27359065
Full Text :
https://doi.org/10.1016/j.biortech.2016.06.078