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Dopamine is Required for Activity-Dependent Amplification of Arc mRNA in Developing Postnatal Frontal Cortex.
- Source :
-
Cerebral cortex (New York, N.Y. : 1991) [Cereb Cortex] 2017 Jul 01; Vol. 27 (7), pp. 3600-3608. - Publication Year :
- 2017
-
Abstract
- The activity-regulated gene Arc/Arg3.1 encodes a postsynaptic protein crucially involved in glutamatergic synaptic plasticity. Genetic mutations in Arc pathway and altered Arc expression in human frontal cortex have been associated with schizophrenia. Although Arc expression has been reported to vary with age, what mechanisms regulate Arc mRNA levels in frontal cortex during postnatal development remains unclear. Using quantitative mRNA analysis of mouse frontal cortical tissues, we mapped the developmental profiles of Arc expression and found that its mRNA levels are sharply amplified near the end of the second postnatal week, when mouse pups open their eyes for the first time after birth. Surprisingly, electrical stimulation of the frontal cortex before eye-opening is not sufficient to drive the amplification of Arc mRNA. Instead, this amplification needs both electrical stimulation and dopamine D1-type receptor (D1R) activation. Furthermore, visual stimuli-driven amplification of Arc mRNA is also dependent on D1R activation and dopamine neurons located in the ventral midbrain. These results indicate that dopamine is required to drive activity-dependent amplification of Arc mRNA in the developing postnatal frontal cortex and suggest that joint electrical and dopaminergic activation is essential to establish the normal expression pattern of a schizophrenia-associated gene during frontal cortical development.<br /> (Published by Oxford University Press 2016.)
- Subjects :
- AIDS-Related Complex metabolism
Adrenergic Agents pharmacology
Age Factors
Amphetamine pharmacology
Animals
Animals, Newborn
Antioxidants pharmacology
Ascorbic Acid pharmacology
Benzazepines pharmacology
Dopamine Agents pharmacology
Dopaminergic Neurons drug effects
Electroshock methods
Mice
Mice, Inbred C57BL
Microarray Analysis
Oxidopamine pharmacology
Receptors, Dopamine D1 metabolism
Ventral Tegmental Area cytology
AIDS-Related Complex genetics
Dopamine metabolism
Frontal Lobe growth & development
Frontal Lobe metabolism
Gene Expression Regulation, Developmental physiology
RNA, Messenger metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2199
- Volume :
- 27
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cerebral cortex (New York, N.Y. : 1991)
- Publication Type :
- Academic Journal
- Accession number :
- 27365296
- Full Text :
- https://doi.org/10.1093/cercor/bhw181