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Comprehensive two-dimensional liquid chromatography-tandem mass spectrometry for the simultaneous determination of wine polyphenols and target contaminants.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2016 Aug 05; Vol. 1458, pp. 54-62. Date of Electronic Publication: 2016 Jun 16. - Publication Year :
- 2016
-
Abstract
- A novel system for comprehensive two-dimensional liquid chromatography coupled to a triple quadrupole mass spectrometer is described for the analysis of wine components. The first dimension consisted of a 250-mm microbore cyano column utilizing 5μm diameter particles, interfaced to a 50-mm superficially-porous particle C18 column with 2.7μm diameter particles. Both columns were operated under reversed-phase conditions. Correlation between the two chromatographic separation modes was decreased by designing a 60-s shift gradient program in the second dimension, and the increase in orthogonality was evaluated quantitatively utilizing a number of orthogonality metrics. The system was employed for the analysis of a red wine sample, without preliminary clean-up procedures, and a total of 43 polyphenols were separated and identified. Comparison with a one-dimensional LC system showed a large increase in the number of identified components with the two-dimensional system. Optimized multiple reaction monitoring experiments allowed for the determination of trans-resveratrol, which is one of the most active antioxidant component of wine, and for monuron, a plant protection product (herbicide) of interest to regulatory agencies. The estimated limits of detection and of quantification were 0.3μgL(-1) and 1μgL(-1), respectively, well below the minimum detection limit (10μgL(-1)) set by current regulation.<br /> (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Subjects :
- Antioxidants analysis
Chromatography, Liquid instrumentation
Food Contamination analysis
Herbicides analysis
Limit of Detection
Methylurea Compounds analysis
Polyphenols chemistry
Resveratrol
Stilbenes analysis
Tandem Mass Spectrometry instrumentation
Chromatography, Liquid methods
Polyphenols analysis
Tandem Mass Spectrometry methods
Wine analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1458
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 27372414
- Full Text :
- https://doi.org/10.1016/j.chroma.2016.06.042